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Blood parameters as biomarkers in a Salmonella spp. disease model of weaning piglets

BACKGROUND: The weaning pig is used as an experimental model to assess the impact of diet on intestinal health. Blood parameters (BP) are considered a useful tool in humans, but there is very scarce information of such indicators in the weaning pig. The objective of the present study is to evaluate...

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Autores principales: Barba-Vidal, Emili, Buttow Roll, Victor Fernando, Garcia Manzanilla, Edgar, Torrente, Carlos, Moreno Muñoz, José Antonio, Pérez, José Francisco, Martín-Orúe, Susana María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658074/
https://www.ncbi.nlm.nih.gov/pubmed/29073182
http://dx.doi.org/10.1371/journal.pone.0186781
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author Barba-Vidal, Emili
Buttow Roll, Victor Fernando
Garcia Manzanilla, Edgar
Torrente, Carlos
Moreno Muñoz, José Antonio
Pérez, José Francisco
Martín-Orúe, Susana María
author_facet Barba-Vidal, Emili
Buttow Roll, Victor Fernando
Garcia Manzanilla, Edgar
Torrente, Carlos
Moreno Muñoz, José Antonio
Pérez, José Francisco
Martín-Orúe, Susana María
author_sort Barba-Vidal, Emili
collection PubMed
description BACKGROUND: The weaning pig is used as an experimental model to assess the impact of diet on intestinal health. Blood parameters (BP) are considered a useful tool in humans, but there is very scarce information of such indicators in the weaning pig. The objective of the present study is to evaluate the use of different BP as indicators in an experimental model of salmonellosis. METHODOLOGY: Seventy-two 28-day-old piglets were divided into four groups in a 2x2 factorial arrangement, with animals receiving or not a probiotic combination based on B. infantis IM1® and B. lactis BPL6 (10(9) colony forming units (cfu)/d) and orally challenged or not a week later with Salmonella Typhimurium (5x10(8) cfu). Blood samples of one animal per pen (N = 24) were taken four days post-inoculation for the evaluation of different BP using an I-stat® System and of plasmatic concentrations of zinc, iron and copper. PRINCIPAL FINDINGS: Results reported marginal deficiencies of zinc in piglets at weaning. Moreover, plasmatic zinc, copper and iron presented good correlations with weight gain (r 0.57, r -0.67, r 0.54 respectively; P < 0.01). Blood electrolytes (Na(+), Cl(-) and K(+)) decreased (P < 0.01) only when the performance of the animals was seriously compromised and clinical symptoms were more apparent. Acid-base balance parameters such as HCO3(-), TCO2 and BE(ecf) significantly correlated with weight gain, but only in the challenged animals (r -0.54, r -0.55, and r -0.51, respectively; P < 0.05), suggesting metabolic acidosis depending on Salmonella infection. Glucose was affected by the challenge (P = 0.040), while Htc and Hgb increased with the challenge and decreased with the probiotic (P < 0.05). Furthermore, correlations of Glu, Htc and Hgb with weight gain were observed (P < 0.05). Overall, BP could be regarded as simple, useful indexes to assess performance and health of weaning piglets.
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spelling pubmed-56580742017-11-09 Blood parameters as biomarkers in a Salmonella spp. disease model of weaning piglets Barba-Vidal, Emili Buttow Roll, Victor Fernando Garcia Manzanilla, Edgar Torrente, Carlos Moreno Muñoz, José Antonio Pérez, José Francisco Martín-Orúe, Susana María PLoS One Research Article BACKGROUND: The weaning pig is used as an experimental model to assess the impact of diet on intestinal health. Blood parameters (BP) are considered a useful tool in humans, but there is very scarce information of such indicators in the weaning pig. The objective of the present study is to evaluate the use of different BP as indicators in an experimental model of salmonellosis. METHODOLOGY: Seventy-two 28-day-old piglets were divided into four groups in a 2x2 factorial arrangement, with animals receiving or not a probiotic combination based on B. infantis IM1® and B. lactis BPL6 (10(9) colony forming units (cfu)/d) and orally challenged or not a week later with Salmonella Typhimurium (5x10(8) cfu). Blood samples of one animal per pen (N = 24) were taken four days post-inoculation for the evaluation of different BP using an I-stat® System and of plasmatic concentrations of zinc, iron and copper. PRINCIPAL FINDINGS: Results reported marginal deficiencies of zinc in piglets at weaning. Moreover, plasmatic zinc, copper and iron presented good correlations with weight gain (r 0.57, r -0.67, r 0.54 respectively; P < 0.01). Blood electrolytes (Na(+), Cl(-) and K(+)) decreased (P < 0.01) only when the performance of the animals was seriously compromised and clinical symptoms were more apparent. Acid-base balance parameters such as HCO3(-), TCO2 and BE(ecf) significantly correlated with weight gain, but only in the challenged animals (r -0.54, r -0.55, and r -0.51, respectively; P < 0.05), suggesting metabolic acidosis depending on Salmonella infection. Glucose was affected by the challenge (P = 0.040), while Htc and Hgb increased with the challenge and decreased with the probiotic (P < 0.05). Furthermore, correlations of Glu, Htc and Hgb with weight gain were observed (P < 0.05). Overall, BP could be regarded as simple, useful indexes to assess performance and health of weaning piglets. Public Library of Science 2017-10-26 /pmc/articles/PMC5658074/ /pubmed/29073182 http://dx.doi.org/10.1371/journal.pone.0186781 Text en © 2017 Barba-Vidal et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Barba-Vidal, Emili
Buttow Roll, Victor Fernando
Garcia Manzanilla, Edgar
Torrente, Carlos
Moreno Muñoz, José Antonio
Pérez, José Francisco
Martín-Orúe, Susana María
Blood parameters as biomarkers in a Salmonella spp. disease model of weaning piglets
title Blood parameters as biomarkers in a Salmonella spp. disease model of weaning piglets
title_full Blood parameters as biomarkers in a Salmonella spp. disease model of weaning piglets
title_fullStr Blood parameters as biomarkers in a Salmonella spp. disease model of weaning piglets
title_full_unstemmed Blood parameters as biomarkers in a Salmonella spp. disease model of weaning piglets
title_short Blood parameters as biomarkers in a Salmonella spp. disease model of weaning piglets
title_sort blood parameters as biomarkers in a salmonella spp. disease model of weaning piglets
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658074/
https://www.ncbi.nlm.nih.gov/pubmed/29073182
http://dx.doi.org/10.1371/journal.pone.0186781
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