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Chemical crosslinking and mass spectrometry to elucidate the topology of integral membrane proteins

Here we made an attempt to obtain partial structural information on the topology of multispan integral membrane proteins of yeast by isolating organellar membranes, removing peripheral membrane proteins at pH 11.5 and introducing chemical crosslinks between vicinal amino acids either using homo- or...

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Detalles Bibliográficos
Autores principales:	Debelyy, Mykhaylo O., Waridel, Patrice, Quadroni, Manfredo, Schneiter, Roger, Conzelmann, Andreas
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658093/ https://www.ncbi.nlm.nih.gov/pubmed/29073188 http://dx.doi.org/10.1371/journal.pone.0186840

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