Larval honey bees infected with Nosema ceranae have increased vitellogenin titers as young adults

Nosema ceranae is a pervasive and widespread honey bee pathogen that is associated with colony declines and has recently been shown to infect larval honey bees. In adult bees, Nosema infection is known to alter levels of a key protein, vitellogenin (Vg), which is necessary for egg-laying in queens, brood food production in workers, and proper immune function in all female bees. We therefore tested the effects of larval worker infection on hemolymph Vg titers. In 1-day old adult workers that were infected as larvae with 10,000 (10 K) or 40,000 (40 K) live N. ceranae spores/bee, Vg titers were significantly elevated by + 83% and + 73%, respectively, as compared to controls. At 7 days of adult age, Vg remained significantly elevated (+ 68%) in 10 K treated workers as compared to control workers. Nosema infection decreased total hemolymph protein titers in 1 and 7-day old adult bees (−50% in the 10 K and 40 K treated bees). Bees infected as larvae also had a more queen-like sting morphology. They developed slightly but significantly fewer barbs on their stings (−7% in the 40K-treated bees). Higher Vg levels are associated with younger bees. Thus, elevated Vg levels could delay normal age polyethism and disrupt colony balance.