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Evaluation of mouse red blood cell and platelet counting with an automated hematology analyzer
An evaluation of mouse red blood cell (RBC) and platelet (PLT) counting with an automated hematology analyzer was performed with three strains of mice, C57BL/6 (B6), BALB/c (BALB) and DBA/2 (D2). There were no significant differences in RBC and PLT counts between manual and automated optical methods...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658564/ https://www.ncbi.nlm.nih.gov/pubmed/28845024 http://dx.doi.org/10.1292/jvms.17-0387 |
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author | FUKUDA, Teruko ASOU, Eri NOGI, Kimiko GOTO, Kazuo |
author_facet | FUKUDA, Teruko ASOU, Eri NOGI, Kimiko GOTO, Kazuo |
author_sort | FUKUDA, Teruko |
collection | PubMed |
description | An evaluation of mouse red blood cell (RBC) and platelet (PLT) counting with an automated hematology analyzer was performed with three strains of mice, C57BL/6 (B6), BALB/c (BALB) and DBA/2 (D2). There were no significant differences in RBC and PLT counts between manual and automated optical methods in any of the samples, except for D2 mice. For D2, RBC counts obtained using the manual method were significantly lower than those obtained using the automated optical method (P<0.05), and PLT counts obtained using the manual method were higher than those obtained using the automated optical method (P<0.05). An automated hematology analyzer can be used for RBC and PLT counting; however, an appropriate method should be selected when D2 mice samples are used. |
format | Online Article Text |
id | pubmed-5658564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56585642017-11-01 Evaluation of mouse red blood cell and platelet counting with an automated hematology analyzer FUKUDA, Teruko ASOU, Eri NOGI, Kimiko GOTO, Kazuo J Vet Med Sci Laboratory Animal Science An evaluation of mouse red blood cell (RBC) and platelet (PLT) counting with an automated hematology analyzer was performed with three strains of mice, C57BL/6 (B6), BALB/c (BALB) and DBA/2 (D2). There were no significant differences in RBC and PLT counts between manual and automated optical methods in any of the samples, except for D2 mice. For D2, RBC counts obtained using the manual method were significantly lower than those obtained using the automated optical method (P<0.05), and PLT counts obtained using the manual method were higher than those obtained using the automated optical method (P<0.05). An automated hematology analyzer can be used for RBC and PLT counting; however, an appropriate method should be selected when D2 mice samples are used. The Japanese Society of Veterinary Science 2017-08-27 2017-10 /pmc/articles/PMC5658564/ /pubmed/28845024 http://dx.doi.org/10.1292/jvms.17-0387 Text en ©2017 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Laboratory Animal Science FUKUDA, Teruko ASOU, Eri NOGI, Kimiko GOTO, Kazuo Evaluation of mouse red blood cell and platelet counting with an automated hematology analyzer |
title | Evaluation of mouse red blood cell and platelet counting with an automated
hematology analyzer |
title_full | Evaluation of mouse red blood cell and platelet counting with an automated
hematology analyzer |
title_fullStr | Evaluation of mouse red blood cell and platelet counting with an automated
hematology analyzer |
title_full_unstemmed | Evaluation of mouse red blood cell and platelet counting with an automated
hematology analyzer |
title_short | Evaluation of mouse red blood cell and platelet counting with an automated
hematology analyzer |
title_sort | evaluation of mouse red blood cell and platelet counting with an automated
hematology analyzer |
topic | Laboratory Animal Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658564/ https://www.ncbi.nlm.nih.gov/pubmed/28845024 http://dx.doi.org/10.1292/jvms.17-0387 |
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