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Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements

Organic solvent‐tolerant bacteria are outstanding and versatile hosts for the bio‐based production of a broad range of generally toxic aromatic compounds. The energetically costly solvent tolerance mechanisms are subject to multiple levels of regulation, involving among other mobile genetic elements...

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Autores principales: Hosseini, Rohola, Kuepper, Jannis, Koebbing, Sebastian, Blank, Lars M., Wierckx, Nick, de Winde, Johannes H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658596/
https://www.ncbi.nlm.nih.gov/pubmed/28401676
http://dx.doi.org/10.1111/1751-7915.12495
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author Hosseini, Rohola
Kuepper, Jannis
Koebbing, Sebastian
Blank, Lars M.
Wierckx, Nick
de Winde, Johannes H.
author_facet Hosseini, Rohola
Kuepper, Jannis
Koebbing, Sebastian
Blank, Lars M.
Wierckx, Nick
de Winde, Johannes H.
author_sort Hosseini, Rohola
collection PubMed
description Organic solvent‐tolerant bacteria are outstanding and versatile hosts for the bio‐based production of a broad range of generally toxic aromatic compounds. The energetically costly solvent tolerance mechanisms are subject to multiple levels of regulation, involving among other mobile genetic elements. The genome of the solvent‐tolerant Pseudomonas putida S12 contains many such mobile elements that play a major role in the regulation and adaptation to various stress conditions, including the regulation of expression of the solvent efflux pump SrpABC. We recently sequenced the genome of P. putida S12. Detailed annotation identified a threefold higher copy number of the mobile element ISS12 in contrast to earlier observations. In this study, we describe the mobile genetic elements and elaborate on the role of ISS12 in the establishment and maintenance of solvent tolerance in P. putida. We identified three different variants of ISS12 of which a single variant exhibits a high translocation rate. One copy of this variant caused a loss of solvent tolerance in the sequenced strain by disruption of srpA. Solvent tolerance could be restored by applying selective pressure, leading to a clean excision of the mobile element.
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spelling pubmed-56585962017-11-01 Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements Hosseini, Rohola Kuepper, Jannis Koebbing, Sebastian Blank, Lars M. Wierckx, Nick de Winde, Johannes H. Microb Biotechnol Research Articles Organic solvent‐tolerant bacteria are outstanding and versatile hosts for the bio‐based production of a broad range of generally toxic aromatic compounds. The energetically costly solvent tolerance mechanisms are subject to multiple levels of regulation, involving among other mobile genetic elements. The genome of the solvent‐tolerant Pseudomonas putida S12 contains many such mobile elements that play a major role in the regulation and adaptation to various stress conditions, including the regulation of expression of the solvent efflux pump SrpABC. We recently sequenced the genome of P. putida S12. Detailed annotation identified a threefold higher copy number of the mobile element ISS12 in contrast to earlier observations. In this study, we describe the mobile genetic elements and elaborate on the role of ISS12 in the establishment and maintenance of solvent tolerance in P. putida. We identified three different variants of ISS12 of which a single variant exhibits a high translocation rate. One copy of this variant caused a loss of solvent tolerance in the sequenced strain by disruption of srpA. Solvent tolerance could be restored by applying selective pressure, leading to a clean excision of the mobile element. John Wiley and Sons Inc. 2017-04-11 /pmc/articles/PMC5658596/ /pubmed/28401676 http://dx.doi.org/10.1111/1751-7915.12495 Text en © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Hosseini, Rohola
Kuepper, Jannis
Koebbing, Sebastian
Blank, Lars M.
Wierckx, Nick
de Winde, Johannes H.
Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements
title Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements
title_full Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements
title_fullStr Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements
title_full_unstemmed Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements
title_short Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements
title_sort regulation of solvent tolerance in pseudomonas putida s12 mediated by mobile elements
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658596/
https://www.ncbi.nlm.nih.gov/pubmed/28401676
http://dx.doi.org/10.1111/1751-7915.12495
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