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Polyhydroxyalkanoate‐associated phasins as phylogenetically heterogeneous, multipurpose proteins

Polyhydroxyalkanoates (PHAs) are natural polyesters of increasing biotechnological importance that are synthesized by many prokaryotic organisms as carbon and energy storage compounds in limiting growth conditions. PHAs accumulate intracellularly in form of inclusion bodies that are covered with a p...

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Detalles Bibliográficos
Autores principales: Maestro, Beatriz, Sanz, Jesús M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658603/
https://www.ncbi.nlm.nih.gov/pubmed/28425176
http://dx.doi.org/10.1111/1751-7915.12718
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author Maestro, Beatriz
Sanz, Jesús M.
author_facet Maestro, Beatriz
Sanz, Jesús M.
author_sort Maestro, Beatriz
collection PubMed
description Polyhydroxyalkanoates (PHAs) are natural polyesters of increasing biotechnological importance that are synthesized by many prokaryotic organisms as carbon and energy storage compounds in limiting growth conditions. PHAs accumulate intracellularly in form of inclusion bodies that are covered with a proteinaceous surface layer (granule‐associated proteins or GAPs) conforming a network‐like surface of structural, metabolic and regulatory polypeptides, and configuring the PHA granules as complex and well‐organized subcellular structures that have been designated as ‘carbonosomes’. GAPs include several enzymes related to PHA metabolism (synthases, depolymerases and hydroxylases) together with the so‐called phasins, an heterogeneous group of small‐size proteins that cover most of the PHA granule and that are devoid of catalytic functions but nevertheless play an essential role in granule structure and PHA metabolism. Structurally, phasins are amphiphilic proteins that shield the hydrophobic polymer from the cytoplasm. Here, we summarize the characteristics of the different phasins identified so far from PHA producer organisms and highlight the diverse opportunities that they offer in the Biotechnology field.
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spelling pubmed-56586032017-11-01 Polyhydroxyalkanoate‐associated phasins as phylogenetically heterogeneous, multipurpose proteins Maestro, Beatriz Sanz, Jesús M. Microb Biotechnol Minireviews Polyhydroxyalkanoates (PHAs) are natural polyesters of increasing biotechnological importance that are synthesized by many prokaryotic organisms as carbon and energy storage compounds in limiting growth conditions. PHAs accumulate intracellularly in form of inclusion bodies that are covered with a proteinaceous surface layer (granule‐associated proteins or GAPs) conforming a network‐like surface of structural, metabolic and regulatory polypeptides, and configuring the PHA granules as complex and well‐organized subcellular structures that have been designated as ‘carbonosomes’. GAPs include several enzymes related to PHA metabolism (synthases, depolymerases and hydroxylases) together with the so‐called phasins, an heterogeneous group of small‐size proteins that cover most of the PHA granule and that are devoid of catalytic functions but nevertheless play an essential role in granule structure and PHA metabolism. Structurally, phasins are amphiphilic proteins that shield the hydrophobic polymer from the cytoplasm. Here, we summarize the characteristics of the different phasins identified so far from PHA producer organisms and highlight the diverse opportunities that they offer in the Biotechnology field. John Wiley and Sons Inc. 2017-04-20 /pmc/articles/PMC5658603/ /pubmed/28425176 http://dx.doi.org/10.1111/1751-7915.12718 Text en © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Minireviews
Maestro, Beatriz
Sanz, Jesús M.
Polyhydroxyalkanoate‐associated phasins as phylogenetically heterogeneous, multipurpose proteins
title Polyhydroxyalkanoate‐associated phasins as phylogenetically heterogeneous, multipurpose proteins
title_full Polyhydroxyalkanoate‐associated phasins as phylogenetically heterogeneous, multipurpose proteins
title_fullStr Polyhydroxyalkanoate‐associated phasins as phylogenetically heterogeneous, multipurpose proteins
title_full_unstemmed Polyhydroxyalkanoate‐associated phasins as phylogenetically heterogeneous, multipurpose proteins
title_short Polyhydroxyalkanoate‐associated phasins as phylogenetically heterogeneous, multipurpose proteins
title_sort polyhydroxyalkanoate‐associated phasins as phylogenetically heterogeneous, multipurpose proteins
topic Minireviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658603/
https://www.ncbi.nlm.nih.gov/pubmed/28425176
http://dx.doi.org/10.1111/1751-7915.12718
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