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Genetic engineering of Trichoderma reesei cellulases and their production
Lignocellulosic biomass, which mainly consists of cellulose, hemicellulose and lignin, is the most abundant renewable source for production of biofuel and biorefinery products. The industrial use of plant biomass involves mechanical milling or chipping, followed by chemical or physicochemical pretre...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658622/ https://www.ncbi.nlm.nih.gov/pubmed/28557371 http://dx.doi.org/10.1111/1751-7915.12726 |
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author | Druzhinina, Irina S. Kubicek, Christian P. |
author_facet | Druzhinina, Irina S. Kubicek, Christian P. |
author_sort | Druzhinina, Irina S. |
collection | PubMed |
description | Lignocellulosic biomass, which mainly consists of cellulose, hemicellulose and lignin, is the most abundant renewable source for production of biofuel and biorefinery products. The industrial use of plant biomass involves mechanical milling or chipping, followed by chemical or physicochemical pretreatment steps to make the material more susceptible to enzymatic hydrolysis. Thereby the cost of enzyme production still presents the major bottleneck, mostly because some of the produced enzymes have low catalytic activity under industrial conditions and/or because the rate of hydrolysis of some enzymes in the secreted enzyme mixture is limiting. Almost all of the lignocellulolytic enzyme cocktails needed for the hydrolysis step are produced by fermentation of the ascomycete Trichoderma reesei (Hypocreales). For this reason, the structure and mechanism of the enzymes involved, the regulation of their expression and the pathways of their formation and secretion have been investigated in T. reesei in considerable details. Several of the findings thereby obtained have been used to improve the formation of the T. reesei cellulases and their properties. In this article, we will review the achievements that have already been made and also show promising fields for further progress. |
format | Online Article Text |
id | pubmed-5658622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56586222017-11-01 Genetic engineering of Trichoderma reesei cellulases and their production Druzhinina, Irina S. Kubicek, Christian P. Microb Biotechnol Minireviews Lignocellulosic biomass, which mainly consists of cellulose, hemicellulose and lignin, is the most abundant renewable source for production of biofuel and biorefinery products. The industrial use of plant biomass involves mechanical milling or chipping, followed by chemical or physicochemical pretreatment steps to make the material more susceptible to enzymatic hydrolysis. Thereby the cost of enzyme production still presents the major bottleneck, mostly because some of the produced enzymes have low catalytic activity under industrial conditions and/or because the rate of hydrolysis of some enzymes in the secreted enzyme mixture is limiting. Almost all of the lignocellulolytic enzyme cocktails needed for the hydrolysis step are produced by fermentation of the ascomycete Trichoderma reesei (Hypocreales). For this reason, the structure and mechanism of the enzymes involved, the regulation of their expression and the pathways of their formation and secretion have been investigated in T. reesei in considerable details. Several of the findings thereby obtained have been used to improve the formation of the T. reesei cellulases and their properties. In this article, we will review the achievements that have already been made and also show promising fields for further progress. John Wiley and Sons Inc. 2017-05-29 /pmc/articles/PMC5658622/ /pubmed/28557371 http://dx.doi.org/10.1111/1751-7915.12726 Text en © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Minireviews Druzhinina, Irina S. Kubicek, Christian P. Genetic engineering of Trichoderma reesei cellulases and their production |
title | Genetic engineering of Trichoderma reesei cellulases and their production |
title_full | Genetic engineering of Trichoderma reesei cellulases and their production |
title_fullStr | Genetic engineering of Trichoderma reesei cellulases and their production |
title_full_unstemmed | Genetic engineering of Trichoderma reesei cellulases and their production |
title_short | Genetic engineering of Trichoderma reesei cellulases and their production |
title_sort | genetic engineering of trichoderma reesei cellulases and their production |
topic | Minireviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658622/ https://www.ncbi.nlm.nih.gov/pubmed/28557371 http://dx.doi.org/10.1111/1751-7915.12726 |
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