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Expression of and correlation between BCL6 and ZEB family members in patients with breast cancer

B-cell lymphoma 6 (BCL6), a proto-oncogene, is an evolutionarily conserved zinc finger protein that functions as a transcriptional repressor. BCL6 is the master regulator of B-lymphocyte development, and it has been reported that BCL6 may serve an important role in breast cancer progression. The aim...

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Autores principales: Ang, Lin, Zheng, Li, Wang, Jin, Huang, Jin, Hu, Hong-Guang, Zou, Qiang, Zhao, Yang, Liu, Qiang-Ming, Zhao, Min, Wu, Zheng-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658690/
https://www.ncbi.nlm.nih.gov/pubmed/29104620
http://dx.doi.org/10.3892/etm.2017.5101
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author Ang, Lin
Zheng, Li
Wang, Jin
Huang, Jin
Hu, Hong-Guang
Zou, Qiang
Zhao, Yang
Liu, Qiang-Ming
Zhao, Min
Wu, Zheng-Sheng
author_facet Ang, Lin
Zheng, Li
Wang, Jin
Huang, Jin
Hu, Hong-Guang
Zou, Qiang
Zhao, Yang
Liu, Qiang-Ming
Zhao, Min
Wu, Zheng-Sheng
author_sort Ang, Lin
collection PubMed
description B-cell lymphoma 6 (BCL6), a proto-oncogene, is an evolutionarily conserved zinc finger protein that functions as a transcriptional repressor. BCL6 is the master regulator of B-lymphocyte development, and it has been reported that BCL6 may serve an important role in breast cancer progression. The aim of the present study was to investigate the expression of BCL6, zinc finger E-box-binding homeobox (ZEB)1 and ZEB2 and their associations in breast cancer. The mRNA and protein expression of BCL6, ZEB1 and ZEB2 was assessed using in situ hybridization and immunohistochemistry, respectively, in 228 patients with breast cancer and 80 patients with benign breast disease. In addition, the association between BCL6, ZEB1 and ZEB2 expression and the clinicopathological characteristics and survival of patients with breast cancer were analyzed. The mRNA and protein expression of BCL6, ZEB1 and ZEB2 were significantly higher in breast cancer tissues compared with benign breast disease tissues (P<0.05). The expression of BCL6, ZEB1 and ZEB2 were significantly positively correlated with tumor size, lymph node metastasis and a higher tumor stage (P<0.05). Furthermore, patients with BCL6, ZEB1 and ZEB2 protein-positive primary tumors had significantly lower overall survival (P=0.001, 0.002 and 0.001, respectively) and relapse-free survival (P=0.002, 0.001 and 0.003, respectively) rates. The mRNA expressions of ZEB1 (r(s)=0.326, P<0.001) and ZEB2 (r(s)=0.382, P<0.001) were significantly positively correlated with BCL6 mRNA expression, and the protein expressions of ZEB1 ((r(s)=0.449, P<0.001) and ZEB2 (r(s)=0.669, P<0.001) were significantly positively correlated with BCL6 protein expression. These results suggest that BCL6, ZEB1 and ZEB2 are potential biomarkers for the invasion, metastasis and prognosis of breast cancer, and that BCL6 may be a regulator of the ZEB family.
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spelling pubmed-56586902017-11-04 Expression of and correlation between BCL6 and ZEB family members in patients with breast cancer Ang, Lin Zheng, Li Wang, Jin Huang, Jin Hu, Hong-Guang Zou, Qiang Zhao, Yang Liu, Qiang-Ming Zhao, Min Wu, Zheng-Sheng Exp Ther Med Articles B-cell lymphoma 6 (BCL6), a proto-oncogene, is an evolutionarily conserved zinc finger protein that functions as a transcriptional repressor. BCL6 is the master regulator of B-lymphocyte development, and it has been reported that BCL6 may serve an important role in breast cancer progression. The aim of the present study was to investigate the expression of BCL6, zinc finger E-box-binding homeobox (ZEB)1 and ZEB2 and their associations in breast cancer. The mRNA and protein expression of BCL6, ZEB1 and ZEB2 was assessed using in situ hybridization and immunohistochemistry, respectively, in 228 patients with breast cancer and 80 patients with benign breast disease. In addition, the association between BCL6, ZEB1 and ZEB2 expression and the clinicopathological characteristics and survival of patients with breast cancer were analyzed. The mRNA and protein expression of BCL6, ZEB1 and ZEB2 were significantly higher in breast cancer tissues compared with benign breast disease tissues (P<0.05). The expression of BCL6, ZEB1 and ZEB2 were significantly positively correlated with tumor size, lymph node metastasis and a higher tumor stage (P<0.05). Furthermore, patients with BCL6, ZEB1 and ZEB2 protein-positive primary tumors had significantly lower overall survival (P=0.001, 0.002 and 0.001, respectively) and relapse-free survival (P=0.002, 0.001 and 0.003, respectively) rates. The mRNA expressions of ZEB1 (r(s)=0.326, P<0.001) and ZEB2 (r(s)=0.382, P<0.001) were significantly positively correlated with BCL6 mRNA expression, and the protein expressions of ZEB1 ((r(s)=0.449, P<0.001) and ZEB2 (r(s)=0.669, P<0.001) were significantly positively correlated with BCL6 protein expression. These results suggest that BCL6, ZEB1 and ZEB2 are potential biomarkers for the invasion, metastasis and prognosis of breast cancer, and that BCL6 may be a regulator of the ZEB family. D.A. Spandidos 2017-11 2017-09-05 /pmc/articles/PMC5658690/ /pubmed/29104620 http://dx.doi.org/10.3892/etm.2017.5101 Text en Copyright: © Ang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ang, Lin
Zheng, Li
Wang, Jin
Huang, Jin
Hu, Hong-Guang
Zou, Qiang
Zhao, Yang
Liu, Qiang-Ming
Zhao, Min
Wu, Zheng-Sheng
Expression of and correlation between BCL6 and ZEB family members in patients with breast cancer
title Expression of and correlation between BCL6 and ZEB family members in patients with breast cancer
title_full Expression of and correlation between BCL6 and ZEB family members in patients with breast cancer
title_fullStr Expression of and correlation between BCL6 and ZEB family members in patients with breast cancer
title_full_unstemmed Expression of and correlation between BCL6 and ZEB family members in patients with breast cancer
title_short Expression of and correlation between BCL6 and ZEB family members in patients with breast cancer
title_sort expression of and correlation between bcl6 and zeb family members in patients with breast cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658690/
https://www.ncbi.nlm.nih.gov/pubmed/29104620
http://dx.doi.org/10.3892/etm.2017.5101
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