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A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity
A peptide has been designed so that its chelating affinity for one type of metal ion regulates its affinity for a second, different type of metal ion. The prochelator peptide (PCP), which is a fusion of motifs evocative of calcium loops and zinc fingers, forms a 1 : 2 Zn : peptide complex at pH 7.4...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5659173/ https://www.ncbi.nlm.nih.gov/pubmed/29511523 http://dx.doi.org/10.1039/c5sc00602c |
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author | Alies, Bruno Wiener, Jacob D. Franz, Katherine J. |
author_facet | Alies, Bruno Wiener, Jacob D. Franz, Katherine J. |
author_sort | Alies, Bruno |
collection | PubMed |
description | A peptide has been designed so that its chelating affinity for one type of metal ion regulates its affinity for a second, different type of metal ion. The prochelator peptide (PCP), which is a fusion of motifs evocative of calcium loops and zinc fingers, forms a 1 : 2 Zn : peptide complex at pH 7.4 that increases its affinity for Zn(2+) ∼3-fold in the presence of Tb(3+) (log β(2) from 13.8 to 14.3), while the 1 : 1 luminescent complex with Tb(3+) is brighter, longer lived, and 20-fold tighter in the presence of Zn(2+) (log K from 6.2 to 7.5). This unique example of cooperative, heterometallic allostery in a biologically compatible construct suggests the possibility of designing conditionally active metal-binding agents that could respond to dynamic changes in cellular metal status. |
format | Online Article Text |
id | pubmed-5659173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-56591732018-03-06 A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity Alies, Bruno Wiener, Jacob D. Franz, Katherine J. Chem Sci Chemistry A peptide has been designed so that its chelating affinity for one type of metal ion regulates its affinity for a second, different type of metal ion. The prochelator peptide (PCP), which is a fusion of motifs evocative of calcium loops and zinc fingers, forms a 1 : 2 Zn : peptide complex at pH 7.4 that increases its affinity for Zn(2+) ∼3-fold in the presence of Tb(3+) (log β(2) from 13.8 to 14.3), while the 1 : 1 luminescent complex with Tb(3+) is brighter, longer lived, and 20-fold tighter in the presence of Zn(2+) (log K from 6.2 to 7.5). This unique example of cooperative, heterometallic allostery in a biologically compatible construct suggests the possibility of designing conditionally active metal-binding agents that could respond to dynamic changes in cellular metal status. Royal Society of Chemistry 2015-06-01 2015-05-05 /pmc/articles/PMC5659173/ /pubmed/29511523 http://dx.doi.org/10.1039/c5sc00602c Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0) |
spellingShingle | Chemistry Alies, Bruno Wiener, Jacob D. Franz, Katherine J. A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity |
title | A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity
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title_full | A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity
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title_fullStr | A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity
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title_full_unstemmed | A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity
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title_short | A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity
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title_sort | prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5659173/ https://www.ncbi.nlm.nih.gov/pubmed/29511523 http://dx.doi.org/10.1039/c5sc00602c |
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