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Real-time observation of polymerase-promoter contact remodeling during transcription initiation

Critical contacts made between the RNA polymerase (RNAP) holoenzyme and promoter DNA modulate not only the strength of promoter binding, but also the frequency and timing of promoter escape during transcription. Here, we describe a single-molecule optical-trapping assay to study transcription initia...

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Detalles Bibliográficos
Autores principales:	Meng, Cong A., Fazal, Furqan M., Block, Steven M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660091/ https://www.ncbi.nlm.nih.gov/pubmed/29079833 http://dx.doi.org/10.1038/s41467-017-01041-1

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