Fluorescence-based cell-specific detection for laser-capture microdissection in human brain

Cell-specific molecular investigations of the human brain are essential for understanding the neurobiology of diseases, but are hindered by postmortem conditions and technical challenges. To address these issues we developed a multi-label fluorescence in situ hybridization protocol and a novel optic...

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Autores principales: Rocco, Brad R., Oh, Hyunjung, Shukla, Rammohan, Mechawar, Naguib, Sibille, Etienne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660154/
https://www.ncbi.nlm.nih.gov/pubmed/29079825
http://dx.doi.org/10.1038/s41598-017-14484-9
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author Rocco, Brad R.
Oh, Hyunjung
Shukla, Rammohan
Mechawar, Naguib
Sibille, Etienne
author_facet Rocco, Brad R.
Oh, Hyunjung
Shukla, Rammohan
Mechawar, Naguib
Sibille, Etienne
author_sort Rocco, Brad R.
collection PubMed
description Cell-specific molecular investigations of the human brain are essential for understanding the neurobiology of diseases, but are hindered by postmortem conditions and technical challenges. To address these issues we developed a multi-label fluorescence in situ hybridization protocol and a novel optical filter device to identify cell types and control for tissue autofluorescence. We show that these methods can be used with laser-capture microdissection for human brain tissue cell-specific molecular analysis.
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spelling pubmed-56601542017-11-01 Fluorescence-based cell-specific detection for laser-capture microdissection in human brain Rocco, Brad R. Oh, Hyunjung Shukla, Rammohan Mechawar, Naguib Sibille, Etienne Sci Rep Article Cell-specific molecular investigations of the human brain are essential for understanding the neurobiology of diseases, but are hindered by postmortem conditions and technical challenges. To address these issues we developed a multi-label fluorescence in situ hybridization protocol and a novel optical filter device to identify cell types and control for tissue autofluorescence. We show that these methods can be used with laser-capture microdissection for human brain tissue cell-specific molecular analysis. Nature Publishing Group UK 2017-10-27 /pmc/articles/PMC5660154/ /pubmed/29079825 http://dx.doi.org/10.1038/s41598-017-14484-9 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Rocco, Brad R.
Oh, Hyunjung
Shukla, Rammohan
Mechawar, Naguib
Sibille, Etienne
Fluorescence-based cell-specific detection for laser-capture microdissection in human brain
title Fluorescence-based cell-specific detection for laser-capture microdissection in human brain
title_full Fluorescence-based cell-specific detection for laser-capture microdissection in human brain
title_fullStr Fluorescence-based cell-specific detection for laser-capture microdissection in human brain
title_full_unstemmed Fluorescence-based cell-specific detection for laser-capture microdissection in human brain
title_short Fluorescence-based cell-specific detection for laser-capture microdissection in human brain
title_sort fluorescence-based cell-specific detection for laser-capture microdissection in human brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660154/
https://www.ncbi.nlm.nih.gov/pubmed/29079825
http://dx.doi.org/10.1038/s41598-017-14484-9
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