Cargando…
In Vitro Seamless Stack Enzymatic Assembly of DNA Molecules Based on a Strategy Involving Splicing of Restriction Sites
The standard binary enzymatic assembly, which operates by inserting one DNA fragment into a plasmid, has a higher assembly success rate than the polynary enzymatic assembly, which inserts two or more fragments into the plasmid. However, it often leaves a nucleotide scar at the junction site. When a...
Autores principales: | Yu, Dong, Tan, Yanning, Sun, Zhizhong, Sun, Xuewu, Sheng, Xiabing, Zhou, Tianshun, Liu, Ling, Mo, Yi, Jiang, Beibei, Ouyang, Ning, Yin, Xiaolin, Duan, Meijuan, Yuan, Dingyang |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660187/ https://www.ncbi.nlm.nih.gov/pubmed/29079784 http://dx.doi.org/10.1038/s41598-017-14496-5 |
Ejemplares similares
-
Cyclic Digestion and Ligation-Mediated PCR Used for Flanking Sequence Walking
por: Yu, Dong, et al.
Publicado: (2020) -
Conversion of a rice CMS maintainer into a photo- or thermo-sensitive genetic male sterile line
por: Tan, Yanning, et al.
Publicado: (2018) -
QTL analysis and dissection of panicle components in rice using advanced backcross populations derived from Oryza Sativa cultivars HR1128 and ‘Nipponbare’
por: Sun, Zhizhong, et al.
Publicado: (2017) -
The Cds.71 on TMS5 May Act as a Mutation Hotspot to Originate a TGMS Trait in Indica Rice Cultivars
por: Tan, Yanning, et al.
Publicado: (2020) -
Improvement of the Rice “Easy-to-Shatter” Trait via CRISPR/Cas9-Mediated Mutagenesis of the qSH1 Gene
por: Sheng, Xiabing, et al.
Publicado: (2020)