Differential gene expression of Australian Cricotopus draysoni (Diptera: Chironomidae) populations reveals seasonal association in detoxification gene regulation

Understanding the molecular mechanisms of organismal response to human-derived ecosystem change is recognised as a critical tool in monitoring and managing impacts, especially in freshwater systems. Fundamental to this approach is to determine the genes involved in responding to ecosystem change and detect modifications to their expression and activity in natural populations. Potential targets for this approach include well-known detoxification genes that are upregulated in response to stress. Here, we tested whether expression of such genes varied in association with differences in ecosystem health and could be detected in the field. We sampled populations of the freshwater midge, Cricotopus draysoni, from two geographically proximate sites in southeast Queensland, Australia, which differed in their ecosystem health, at multiple time points. We assessed transcriptome-level differential gene expression and predicted greatest differential expression between sites, associated with organismal responses to local physico-chemical factors. In contrast, we observed a clear and dramatic difference in gene expression – including of known detoxification genes – between time points, specifically between periods at the start and end of the austral summer rainfall when in-stream water levels are most different. These data suggest that these waterways experience greatest pollution load when water levels are high following rainfall events.