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Re-Evaluation of Imaging Methods of Reactive Oxygen and Nitrogen Species in Plants and Fungi: Influence of Cell Wall Composition
Developmental transitions and stress reactions in both eukaryotes and prokaryotes are tightly linked with fast and localized modifications in concentrations of reactive oxygen and nitrogen species (ROS and RNS). Fluorescent microscopic analyses are widely applied to detect localized production of RO...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660854/ https://www.ncbi.nlm.nih.gov/pubmed/29114232 http://dx.doi.org/10.3389/fphys.2017.00826 |
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author | Sedlářová, Michaela Luhová, Lenka |
author_facet | Sedlářová, Michaela Luhová, Lenka |
author_sort | Sedlářová, Michaela |
collection | PubMed |
description | Developmental transitions and stress reactions in both eukaryotes and prokaryotes are tightly linked with fast and localized modifications in concentrations of reactive oxygen and nitrogen species (ROS and RNS). Fluorescent microscopic analyses are widely applied to detect localized production of ROS and RNS in vivo. In this mini-review we discuss the biological characteristics of studied material (cell wall, extracellular matrix, and tissue complexity) and its handling (concentration of probes, effect of pressure, and higher temperature) which influence results of histochemical staining with “classical” fluorochromes. Future perspectives of ROS and RNS imaging with newly designed probes are briefly outlined. |
format | Online Article Text |
id | pubmed-5660854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56608542017-11-07 Re-Evaluation of Imaging Methods of Reactive Oxygen and Nitrogen Species in Plants and Fungi: Influence of Cell Wall Composition Sedlářová, Michaela Luhová, Lenka Front Physiol Physiology Developmental transitions and stress reactions in both eukaryotes and prokaryotes are tightly linked with fast and localized modifications in concentrations of reactive oxygen and nitrogen species (ROS and RNS). Fluorescent microscopic analyses are widely applied to detect localized production of ROS and RNS in vivo. In this mini-review we discuss the biological characteristics of studied material (cell wall, extracellular matrix, and tissue complexity) and its handling (concentration of probes, effect of pressure, and higher temperature) which influence results of histochemical staining with “classical” fluorochromes. Future perspectives of ROS and RNS imaging with newly designed probes are briefly outlined. Frontiers Media S.A. 2017-10-24 /pmc/articles/PMC5660854/ /pubmed/29114232 http://dx.doi.org/10.3389/fphys.2017.00826 Text en Copyright © 2017 Sedlářová and Luhová. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Sedlářová, Michaela Luhová, Lenka Re-Evaluation of Imaging Methods of Reactive Oxygen and Nitrogen Species in Plants and Fungi: Influence of Cell Wall Composition |
title | Re-Evaluation of Imaging Methods of Reactive Oxygen and Nitrogen Species in Plants and Fungi: Influence of Cell Wall Composition |
title_full | Re-Evaluation of Imaging Methods of Reactive Oxygen and Nitrogen Species in Plants and Fungi: Influence of Cell Wall Composition |
title_fullStr | Re-Evaluation of Imaging Methods of Reactive Oxygen and Nitrogen Species in Plants and Fungi: Influence of Cell Wall Composition |
title_full_unstemmed | Re-Evaluation of Imaging Methods of Reactive Oxygen and Nitrogen Species in Plants and Fungi: Influence of Cell Wall Composition |
title_short | Re-Evaluation of Imaging Methods of Reactive Oxygen and Nitrogen Species in Plants and Fungi: Influence of Cell Wall Composition |
title_sort | re-evaluation of imaging methods of reactive oxygen and nitrogen species in plants and fungi: influence of cell wall composition |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660854/ https://www.ncbi.nlm.nih.gov/pubmed/29114232 http://dx.doi.org/10.3389/fphys.2017.00826 |
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