Cargando…

Silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon

In this paper, we have used spectroscopic and electron microscopic analysis to monitor the time evolution of the silver nanoparticles (Ag NP)–human hemoglobin (Hb) corona formation and to characterize the interaction of the Ag NPs with Hb. The time constants for surface plasmon resonance binding and...

Descripción completa

Detalles Bibliográficos
Autores principales: Bhunia, A. K., Kamilya, T., Saha, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661023/
https://www.ncbi.nlm.nih.gov/pubmed/29142807
http://dx.doi.org/10.1186/s40580-017-0122-1
_version_ 1783274400202620928
author Bhunia, A. K.
Kamilya, T.
Saha, S.
author_facet Bhunia, A. K.
Kamilya, T.
Saha, S.
author_sort Bhunia, A. K.
collection PubMed
description In this paper, we have used spectroscopic and electron microscopic analysis to monitor the time evolution of the silver nanoparticles (Ag NP)–human hemoglobin (Hb) corona formation and to characterize the interaction of the Ag NPs with Hb. The time constants for surface plasmon resonance binding and reorganization are found to be 9.51 and 118.48 min, respectively. The drop of surface charge and the increase of the hydrodynamic diameter indicated the corona of Hb on the Ag NP surface. The auto correlation function is found to broaden with the increasing time of the corona formation. Surface zeta potential revealed that positively charged Hb interact electrostatically with negatively charged Ag NP surfaces. The change in α helix and β sheet depends on the corona formation time. The visualization of the Hb corona from HRTEM showed large number of Hb domains aggregate containing essentially Ag NPs and without Ag NPs. Emission study showed the tertiary deformation, energy transfer, nature of interaction and quenching under three different temperatures. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40580-017-0122-1) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5661023
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Springer Singapore
record_format MEDLINE/PubMed
spelling pubmed-56610232017-11-13 Silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon Bhunia, A. K. Kamilya, T. Saha, S. Nano Converg Research In this paper, we have used spectroscopic and electron microscopic analysis to monitor the time evolution of the silver nanoparticles (Ag NP)–human hemoglobin (Hb) corona formation and to characterize the interaction of the Ag NPs with Hb. The time constants for surface plasmon resonance binding and reorganization are found to be 9.51 and 118.48 min, respectively. The drop of surface charge and the increase of the hydrodynamic diameter indicated the corona of Hb on the Ag NP surface. The auto correlation function is found to broaden with the increasing time of the corona formation. Surface zeta potential revealed that positively charged Hb interact electrostatically with negatively charged Ag NP surfaces. The change in α helix and β sheet depends on the corona formation time. The visualization of the Hb corona from HRTEM showed large number of Hb domains aggregate containing essentially Ag NPs and without Ag NPs. Emission study showed the tertiary deformation, energy transfer, nature of interaction and quenching under three different temperatures. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40580-017-0122-1) contains supplementary material, which is available to authorized users. Springer Singapore 2017-10-30 /pmc/articles/PMC5661023/ /pubmed/29142807 http://dx.doi.org/10.1186/s40580-017-0122-1 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Bhunia, A. K.
Kamilya, T.
Saha, S.
Silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon
title Silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon
title_full Silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon
title_fullStr Silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon
title_full_unstemmed Silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon
title_short Silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon
title_sort silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661023/
https://www.ncbi.nlm.nih.gov/pubmed/29142807
http://dx.doi.org/10.1186/s40580-017-0122-1
work_keys_str_mv AT bhuniaak silvernanoparticlehumanhemoglobininterfacetimeevolutionofthecoronaformationandinteractionphenomenon
AT kamilyat silvernanoparticlehumanhemoglobininterfacetimeevolutionofthecoronaformationandinteractionphenomenon
AT sahas silvernanoparticlehumanhemoglobininterfacetimeevolutionofthecoronaformationandinteractionphenomenon