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Age‐related modulation of angiogenesis‐regulating factors in the swine meniscus

An in‐depth knowledge of the native meniscus morphology and biomechanics in its different areas is essential to develop an engineered tissue. Meniscus is characterized by a great regional variation in extracellular matrix components and in vascularization. Then, the aim of this work was to character...

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Autores principales: Di Giancamillo, Alessia, Deponti, Daniela, Modina, Silvia, Tessaro, Irene, Domeneghini, Cinzia, Peretti, Giuseppe Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661103/
https://www.ncbi.nlm.nih.gov/pubmed/28580627
http://dx.doi.org/10.1111/jcmm.13218
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author Di Giancamillo, Alessia
Deponti, Daniela
Modina, Silvia
Tessaro, Irene
Domeneghini, Cinzia
Peretti, Giuseppe Maria
author_facet Di Giancamillo, Alessia
Deponti, Daniela
Modina, Silvia
Tessaro, Irene
Domeneghini, Cinzia
Peretti, Giuseppe Maria
author_sort Di Giancamillo, Alessia
collection PubMed
description An in‐depth knowledge of the native meniscus morphology and biomechanics in its different areas is essential to develop an engineered tissue. Meniscus is characterized by a great regional variation in extracellular matrix components and in vascularization. Then, the aim of this work was to characterize the expression of factors involved in angiogenesis in different areas during meniscus maturation in pigs. The menisci were removed from the knee joints of neonatal, young and adult pigs, and they were divided into the inner, intermediate and outer areas. Vascular characterization and meniscal maturation were evaluated by immunohistochemistry and Western blot analysis. In particular, expression of the angiogenic factor Vascular Endothelial Growth Factor (VEGF) and the anti‐angiogenic marker Endostatin (ENDO) was analysed, as well as the vascular endothelial cadherin (Ve‐CAD). In addition, expression of Collagen II (COLL II) and SOX9 was examined, as markers of the fibro‐cartilaginous differentiation. Expression of VEGF and Ve‐CAD had a similar pattern in all animals, with a significant increase from the inner to the outer part of the meniscus. Pooling the zones, expression of both proteins was significantly higher in the neonatal meniscus than in young and adult menisci. Conversely, the young meniscus revealed a significantly higher expression of ENDO compared to the neonatal and adult ones. Analysis of tissue maturation markers showed an increase in COLL II and a decrease in SOX9 expression with age. These preliminary data highlight some of the changes that occur in the swine meniscus during growth, in particular the ensemble of regulatory factors involved in angiogenesis.
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spelling pubmed-56611032017-11-02 Age‐related modulation of angiogenesis‐regulating factors in the swine meniscus Di Giancamillo, Alessia Deponti, Daniela Modina, Silvia Tessaro, Irene Domeneghini, Cinzia Peretti, Giuseppe Maria J Cell Mol Med Original Articles An in‐depth knowledge of the native meniscus morphology and biomechanics in its different areas is essential to develop an engineered tissue. Meniscus is characterized by a great regional variation in extracellular matrix components and in vascularization. Then, the aim of this work was to characterize the expression of factors involved in angiogenesis in different areas during meniscus maturation in pigs. The menisci were removed from the knee joints of neonatal, young and adult pigs, and they were divided into the inner, intermediate and outer areas. Vascular characterization and meniscal maturation were evaluated by immunohistochemistry and Western blot analysis. In particular, expression of the angiogenic factor Vascular Endothelial Growth Factor (VEGF) and the anti‐angiogenic marker Endostatin (ENDO) was analysed, as well as the vascular endothelial cadherin (Ve‐CAD). In addition, expression of Collagen II (COLL II) and SOX9 was examined, as markers of the fibro‐cartilaginous differentiation. Expression of VEGF and Ve‐CAD had a similar pattern in all animals, with a significant increase from the inner to the outer part of the meniscus. Pooling the zones, expression of both proteins was significantly higher in the neonatal meniscus than in young and adult menisci. Conversely, the young meniscus revealed a significantly higher expression of ENDO compared to the neonatal and adult ones. Analysis of tissue maturation markers showed an increase in COLL II and a decrease in SOX9 expression with age. These preliminary data highlight some of the changes that occur in the swine meniscus during growth, in particular the ensemble of regulatory factors involved in angiogenesis. John Wiley and Sons Inc. 2017-06-04 2017-11 /pmc/articles/PMC5661103/ /pubmed/28580627 http://dx.doi.org/10.1111/jcmm.13218 Text en © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Di Giancamillo, Alessia
Deponti, Daniela
Modina, Silvia
Tessaro, Irene
Domeneghini, Cinzia
Peretti, Giuseppe Maria
Age‐related modulation of angiogenesis‐regulating factors in the swine meniscus
title Age‐related modulation of angiogenesis‐regulating factors in the swine meniscus
title_full Age‐related modulation of angiogenesis‐regulating factors in the swine meniscus
title_fullStr Age‐related modulation of angiogenesis‐regulating factors in the swine meniscus
title_full_unstemmed Age‐related modulation of angiogenesis‐regulating factors in the swine meniscus
title_short Age‐related modulation of angiogenesis‐regulating factors in the swine meniscus
title_sort age‐related modulation of angiogenesis‐regulating factors in the swine meniscus
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661103/
https://www.ncbi.nlm.nih.gov/pubmed/28580627
http://dx.doi.org/10.1111/jcmm.13218
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