PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts
The biological function of NADPH oxidase (NOX) is the generation of reactive oxygen species (ROS). ROS, primarily arising from oxidative cell metabolism, play a major role in both chronological ageing and photoageing. ROS in extrinsic and intrinsic skin ageing may be assumed to induce the expression...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661253/ https://www.ncbi.nlm.nih.gov/pubmed/28557373 http://dx.doi.org/10.1111/jcmm.13220 |
_version_ | 1783274450572017664 |
---|---|
author | Noh, Eun‐Mi Kim, Jeong‐Mi Hong, On‐Yu Song, Hyun‐Kyung Kim, Jong‐Suk Kwon, Kang‐Beom Lee, Young‐Rae |
author_facet | Noh, Eun‐Mi Kim, Jeong‐Mi Hong, On‐Yu Song, Hyun‐Kyung Kim, Jong‐Suk Kwon, Kang‐Beom Lee, Young‐Rae |
author_sort | Noh, Eun‐Mi |
collection | PubMed |
description | The biological function of NADPH oxidase (NOX) is the generation of reactive oxygen species (ROS). ROS, primarily arising from oxidative cell metabolism, play a major role in both chronological ageing and photoageing. ROS in extrinsic and intrinsic skin ageing may be assumed to induce the expression of matrix metalloproteinases. NADPH oxidase is closely linked with phosphatidylinositol 3‐OH kinase (PI3K) signalling. Protein kinase C (PKC), a downstream molecule of PI3K, is essential for superoxide generation by NADPH oxidase. However, the effect of PTEN and NOX4 in replicative‐aged MMPs expression has not been determined. In this study, we confirmed that inhibition of the PI3K signalling pathway by PTEN gene transfer abolished the NOX‐4 and MMP‐1 expression. Also, NOX‐4 down‐expression of replicative‐aged skin cells abolished the MMP‐1 expression and ROS generation. These results suggest that increase of MMP‐1 expression by replicative‐induced ROS is related to the change in the PTEN and NOX expression. |
format | Online Article Text |
id | pubmed-5661253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56612532017-11-02 PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts Noh, Eun‐Mi Kim, Jeong‐Mi Hong, On‐Yu Song, Hyun‐Kyung Kim, Jong‐Suk Kwon, Kang‐Beom Lee, Young‐Rae J Cell Mol Med Short Communication The biological function of NADPH oxidase (NOX) is the generation of reactive oxygen species (ROS). ROS, primarily arising from oxidative cell metabolism, play a major role in both chronological ageing and photoageing. ROS in extrinsic and intrinsic skin ageing may be assumed to induce the expression of matrix metalloproteinases. NADPH oxidase is closely linked with phosphatidylinositol 3‐OH kinase (PI3K) signalling. Protein kinase C (PKC), a downstream molecule of PI3K, is essential for superoxide generation by NADPH oxidase. However, the effect of PTEN and NOX4 in replicative‐aged MMPs expression has not been determined. In this study, we confirmed that inhibition of the PI3K signalling pathway by PTEN gene transfer abolished the NOX‐4 and MMP‐1 expression. Also, NOX‐4 down‐expression of replicative‐aged skin cells abolished the MMP‐1 expression and ROS generation. These results suggest that increase of MMP‐1 expression by replicative‐induced ROS is related to the change in the PTEN and NOX expression. John Wiley and Sons Inc. 2017-05-30 2017-11 /pmc/articles/PMC5661253/ /pubmed/28557373 http://dx.doi.org/10.1111/jcmm.13220 Text en © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Noh, Eun‐Mi Kim, Jeong‐Mi Hong, On‐Yu Song, Hyun‐Kyung Kim, Jong‐Suk Kwon, Kang‐Beom Lee, Young‐Rae PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts |
title |
PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts |
title_full |
PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts |
title_fullStr |
PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts |
title_full_unstemmed |
PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts |
title_short |
PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts |
title_sort | pten inhibits replicative senescence‐induced mmp‐1 expression by regulating nox4‐mediated ros in human dermal fibroblasts |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661253/ https://www.ncbi.nlm.nih.gov/pubmed/28557373 http://dx.doi.org/10.1111/jcmm.13220 |
work_keys_str_mv | AT noheunmi pteninhibitsreplicativesenescenceinducedmmp1expressionbyregulatingnox4mediatedrosinhumandermalfibroblasts AT kimjeongmi pteninhibitsreplicativesenescenceinducedmmp1expressionbyregulatingnox4mediatedrosinhumandermalfibroblasts AT hongonyu pteninhibitsreplicativesenescenceinducedmmp1expressionbyregulatingnox4mediatedrosinhumandermalfibroblasts AT songhyunkyung pteninhibitsreplicativesenescenceinducedmmp1expressionbyregulatingnox4mediatedrosinhumandermalfibroblasts AT kimjongsuk pteninhibitsreplicativesenescenceinducedmmp1expressionbyregulatingnox4mediatedrosinhumandermalfibroblasts AT kwonkangbeom pteninhibitsreplicativesenescenceinducedmmp1expressionbyregulatingnox4mediatedrosinhumandermalfibroblasts AT leeyoungrae pteninhibitsreplicativesenescenceinducedmmp1expressionbyregulatingnox4mediatedrosinhumandermalfibroblasts |