PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts

The biological function of NADPH oxidase (NOX) is the generation of reactive oxygen species (ROS). ROS, primarily arising from oxidative cell metabolism, play a major role in both chronological ageing and photoageing. ROS in extrinsic and intrinsic skin ageing may be assumed to induce the expression...

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Autores principales: Noh, Eun‐Mi, Kim, Jeong‐Mi, Hong, On‐Yu, Song, Hyun‐Kyung, Kim, Jong‐Suk, Kwon, Kang‐Beom, Lee, Young‐Rae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661253/
https://www.ncbi.nlm.nih.gov/pubmed/28557373
http://dx.doi.org/10.1111/jcmm.13220
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author Noh, Eun‐Mi
Kim, Jeong‐Mi
Hong, On‐Yu
Song, Hyun‐Kyung
Kim, Jong‐Suk
Kwon, Kang‐Beom
Lee, Young‐Rae
author_facet Noh, Eun‐Mi
Kim, Jeong‐Mi
Hong, On‐Yu
Song, Hyun‐Kyung
Kim, Jong‐Suk
Kwon, Kang‐Beom
Lee, Young‐Rae
author_sort Noh, Eun‐Mi
collection PubMed
description The biological function of NADPH oxidase (NOX) is the generation of reactive oxygen species (ROS). ROS, primarily arising from oxidative cell metabolism, play a major role in both chronological ageing and photoageing. ROS in extrinsic and intrinsic skin ageing may be assumed to induce the expression of matrix metalloproteinases. NADPH oxidase is closely linked with phosphatidylinositol 3‐OH kinase (PI3K) signalling. Protein kinase C (PKC), a downstream molecule of PI3K, is essential for superoxide generation by NADPH oxidase. However, the effect of PTEN and NOX4 in replicative‐aged MMPs expression has not been determined. In this study, we confirmed that inhibition of the PI3K signalling pathway by PTEN gene transfer abolished the NOX‐4 and MMP‐1 expression. Also, NOX‐4 down‐expression of replicative‐aged skin cells abolished the MMP‐1 expression and ROS generation. These results suggest that increase of MMP‐1 expression by replicative‐induced ROS is related to the change in the PTEN and NOX expression.
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spelling pubmed-56612532017-11-02 PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts Noh, Eun‐Mi Kim, Jeong‐Mi Hong, On‐Yu Song, Hyun‐Kyung Kim, Jong‐Suk Kwon, Kang‐Beom Lee, Young‐Rae J Cell Mol Med Short Communication The biological function of NADPH oxidase (NOX) is the generation of reactive oxygen species (ROS). ROS, primarily arising from oxidative cell metabolism, play a major role in both chronological ageing and photoageing. ROS in extrinsic and intrinsic skin ageing may be assumed to induce the expression of matrix metalloproteinases. NADPH oxidase is closely linked with phosphatidylinositol 3‐OH kinase (PI3K) signalling. Protein kinase C (PKC), a downstream molecule of PI3K, is essential for superoxide generation by NADPH oxidase. However, the effect of PTEN and NOX4 in replicative‐aged MMPs expression has not been determined. In this study, we confirmed that inhibition of the PI3K signalling pathway by PTEN gene transfer abolished the NOX‐4 and MMP‐1 expression. Also, NOX‐4 down‐expression of replicative‐aged skin cells abolished the MMP‐1 expression and ROS generation. These results suggest that increase of MMP‐1 expression by replicative‐induced ROS is related to the change in the PTEN and NOX expression. John Wiley and Sons Inc. 2017-05-30 2017-11 /pmc/articles/PMC5661253/ /pubmed/28557373 http://dx.doi.org/10.1111/jcmm.13220 Text en © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Noh, Eun‐Mi
Kim, Jeong‐Mi
Hong, On‐Yu
Song, Hyun‐Kyung
Kim, Jong‐Suk
Kwon, Kang‐Beom
Lee, Young‐Rae
PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts
title PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts
title_full PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts
title_fullStr PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts
title_full_unstemmed PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts
title_short PTEN inhibits replicative senescence‐induced MMP‐1 expression by regulating NOX4‐mediated ROS in human dermal fibroblasts
title_sort pten inhibits replicative senescence‐induced mmp‐1 expression by regulating nox4‐mediated ros in human dermal fibroblasts
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661253/
https://www.ncbi.nlm.nih.gov/pubmed/28557373
http://dx.doi.org/10.1111/jcmm.13220
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