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The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis
The relationship between the expression levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 and breast cancer prognosis was studied. Two breast cancer cell lines (MDA-MB-231 and MCF-7) and one human normal breast cell line (HS578Bst) were investigated. Fluorescence real-time reverse transcription...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661385/ https://www.ncbi.nlm.nih.gov/pubmed/29113219 http://dx.doi.org/10.3892/ol.2017.6924 |
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author | Li, Hai Qiu, Zhenwei Li, Feng Wang, Chunlei |
author_facet | Li, Hai Qiu, Zhenwei Li, Feng Wang, Chunlei |
author_sort | Li, Hai |
collection | PubMed |
description | The relationship between the expression levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 and breast cancer prognosis was studied. Two breast cancer cell lines (MDA-MB-231 and MCF-7) and one human normal breast cell line (HS578Bst) were investigated. Fluorescence real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were used to detect cellular mRNA and protein MMP-2 and MMP-9 expression levels. Breast cancer tissue samples from 80 patients and tumor-adjacent normal tissue samples from 40 patients were collected, and MMP-2 and MMP-9 expression in these samples were examined using immunohistochemistry (IHC). The relationship of MMP-2 and MMP-9 expression levels with breast cancer patient clinicopathological parameters and prognosis was analyzed. RT-PCR and western blot results showed that MMP-2 and MMP-9 mRNA and protein expression levels were significantly higher in MDA-MB-231 and MCF-7 cells than in HS578Bst cells. A high expression of MMP-2 and MMP-9 was found in 83.75% (67/80) and 78.75% (63/80) of breast cancer tissue samples, respectively. MMP-2 and MMP-9 expression in breast cancer tissues were significantly different from that in tumor-adjacent normal tissues (p<0.01). MMP-2 and MMP-9 expression levels in breast cancer tissues were correlated with lymph node metastasis and tumor staging. Single factor survival analysis showed that MMP-2 and MMP-9 were factors influencing breast cancer prognosis. MMP-2 and MMP-9 are highly expressed in breast cancer tissues and are closely related to lymph node metastasis and tumor staging. MMP-2 and MMP-9 can be used as reference indices for guiding breast cancer prognosis and treatment. |
format | Online Article Text |
id | pubmed-5661385 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-56613852017-11-06 The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis Li, Hai Qiu, Zhenwei Li, Feng Wang, Chunlei Oncol Lett Articles The relationship between the expression levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 and breast cancer prognosis was studied. Two breast cancer cell lines (MDA-MB-231 and MCF-7) and one human normal breast cell line (HS578Bst) were investigated. Fluorescence real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were used to detect cellular mRNA and protein MMP-2 and MMP-9 expression levels. Breast cancer tissue samples from 80 patients and tumor-adjacent normal tissue samples from 40 patients were collected, and MMP-2 and MMP-9 expression in these samples were examined using immunohistochemistry (IHC). The relationship of MMP-2 and MMP-9 expression levels with breast cancer patient clinicopathological parameters and prognosis was analyzed. RT-PCR and western blot results showed that MMP-2 and MMP-9 mRNA and protein expression levels were significantly higher in MDA-MB-231 and MCF-7 cells than in HS578Bst cells. A high expression of MMP-2 and MMP-9 was found in 83.75% (67/80) and 78.75% (63/80) of breast cancer tissue samples, respectively. MMP-2 and MMP-9 expression in breast cancer tissues were significantly different from that in tumor-adjacent normal tissues (p<0.01). MMP-2 and MMP-9 expression levels in breast cancer tissues were correlated with lymph node metastasis and tumor staging. Single factor survival analysis showed that MMP-2 and MMP-9 were factors influencing breast cancer prognosis. MMP-2 and MMP-9 are highly expressed in breast cancer tissues and are closely related to lymph node metastasis and tumor staging. MMP-2 and MMP-9 can be used as reference indices for guiding breast cancer prognosis and treatment. D.A. Spandidos 2017-11 2017-09-13 /pmc/articles/PMC5661385/ /pubmed/29113219 http://dx.doi.org/10.3892/ol.2017.6924 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Hai Qiu, Zhenwei Li, Feng Wang, Chunlei The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis |
title | The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis |
title_full | The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis |
title_fullStr | The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis |
title_full_unstemmed | The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis |
title_short | The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis |
title_sort | relationship between mmp-2 and mmp-9 expression levels with breast cancer incidence and prognosis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661385/ https://www.ncbi.nlm.nih.gov/pubmed/29113219 http://dx.doi.org/10.3892/ol.2017.6924 |
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