Silencing of semaphorin 3C suppresses cell proliferation and migration in MCF-7 breast cancer cells

Previous studies have suggested that semaphorin 3C (SEMA3C) is involved in the tumorigenesis and metastasis of a number of types of cancer. The aim of the present study was to investigate the role of SEMA3C in the proliferation and migration of MCF-7 breast cancer cells. Small interfering (si)RNA se...

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Detalles Bibliográficos
Autores principales: Zhu, Xiaofang, Zhang, Xiangjian, Ye, Zhiqiang, Chen, Yizuo, Lv, Lin, Zhang, Xiaohua, Hu, Hongye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661468/
https://www.ncbi.nlm.nih.gov/pubmed/29113226
http://dx.doi.org/10.3892/ol.2017.6920
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author Zhu, Xiaofang
Zhang, Xiangjian
Ye, Zhiqiang
Chen, Yizuo
Lv, Lin
Zhang, Xiaohua
Hu, Hongye
author_facet Zhu, Xiaofang
Zhang, Xiangjian
Ye, Zhiqiang
Chen, Yizuo
Lv, Lin
Zhang, Xiaohua
Hu, Hongye
author_sort Zhu, Xiaofang
collection PubMed
description Previous studies have suggested that semaphorin 3C (SEMA3C) is involved in the tumorigenesis and metastasis of a number of types of cancer. The aim of the present study was to investigate the role of SEMA3C in the proliferation and migration of MCF-7 breast cancer cells. Small interfering (si)RNA sequences targeting SEMA3C were constructed and transfected into MCF-7 cells in order to silence the expression of SEMA3C. Cell proliferation and migration were measured using CCK-8 and Transwell assays, respectively. Transfection with SEMA3C siRNA significantly downregulated the expression of SEMA3C in MCF-7 cells, and significantly suppressed cell proliferation and migration. Therefore, SEMA3C-targeted siRNA may be of potential use for the early diagnosis and treatment of breast cancer.
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spelling pubmed-56614682017-11-06 Silencing of semaphorin 3C suppresses cell proliferation and migration in MCF-7 breast cancer cells Zhu, Xiaofang Zhang, Xiangjian Ye, Zhiqiang Chen, Yizuo Lv, Lin Zhang, Xiaohua Hu, Hongye Oncol Lett Articles Previous studies have suggested that semaphorin 3C (SEMA3C) is involved in the tumorigenesis and metastasis of a number of types of cancer. The aim of the present study was to investigate the role of SEMA3C in the proliferation and migration of MCF-7 breast cancer cells. Small interfering (si)RNA sequences targeting SEMA3C were constructed and transfected into MCF-7 cells in order to silence the expression of SEMA3C. Cell proliferation and migration were measured using CCK-8 and Transwell assays, respectively. Transfection with SEMA3C siRNA significantly downregulated the expression of SEMA3C in MCF-7 cells, and significantly suppressed cell proliferation and migration. Therefore, SEMA3C-targeted siRNA may be of potential use for the early diagnosis and treatment of breast cancer. D.A. Spandidos 2017-11 2017-09-08 /pmc/articles/PMC5661468/ /pubmed/29113226 http://dx.doi.org/10.3892/ol.2017.6920 Text en Copyright: © Zhu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhu, Xiaofang
Zhang, Xiangjian
Ye, Zhiqiang
Chen, Yizuo
Lv, Lin
Zhang, Xiaohua
Hu, Hongye
Silencing of semaphorin 3C suppresses cell proliferation and migration in MCF-7 breast cancer cells
title Silencing of semaphorin 3C suppresses cell proliferation and migration in MCF-7 breast cancer cells
title_full Silencing of semaphorin 3C suppresses cell proliferation and migration in MCF-7 breast cancer cells
title_fullStr Silencing of semaphorin 3C suppresses cell proliferation and migration in MCF-7 breast cancer cells
title_full_unstemmed Silencing of semaphorin 3C suppresses cell proliferation and migration in MCF-7 breast cancer cells
title_short Silencing of semaphorin 3C suppresses cell proliferation and migration in MCF-7 breast cancer cells
title_sort silencing of semaphorin 3c suppresses cell proliferation and migration in mcf-7 breast cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661468/
https://www.ncbi.nlm.nih.gov/pubmed/29113226
http://dx.doi.org/10.3892/ol.2017.6920
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