Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages

Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluatio...

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Autores principales: Villela, Anne Drumond, Rodrigues-Junior, Valnês S, Pinto, Antônio Frederico Michel, Falcão, Virgínia Carla de Almeida, Sánchez-Quitian, Zilpa Adriana, Eichler, Paula, Bizarro, Cristiano Valim, Basso, Luiz Augusto, Santos, Diógenes Santiago
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2017
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661903/
https://www.ncbi.nlm.nih.gov/pubmed/29091140
http://dx.doi.org/10.1590/0074-02760170105
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author Villela, Anne Drumond
Rodrigues-Junior, Valnês S
Pinto, Antônio Frederico Michel
Falcão, Virgínia Carla de Almeida
Sánchez-Quitian, Zilpa Adriana
Eichler, Paula
Bizarro, Cristiano Valim
Basso, Luiz Augusto
Santos, Diógenes Santiago
author_facet Villela, Anne Drumond
Rodrigues-Junior, Valnês S
Pinto, Antônio Frederico Michel
Falcão, Virgínia Carla de Almeida
Sánchez-Quitian, Zilpa Adriana
Eichler, Paula
Bizarro, Cristiano Valim
Basso, Luiz Augusto
Santos, Diógenes Santiago
author_sort Villela, Anne Drumond
collection PubMed
description Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence.
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spelling pubmed-56619032017-11-04 Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages Villela, Anne Drumond Rodrigues-Junior, Valnês S Pinto, Antônio Frederico Michel Falcão, Virgínia Carla de Almeida Sánchez-Quitian, Zilpa Adriana Eichler, Paula Bizarro, Cristiano Valim Basso, Luiz Augusto Santos, Diógenes Santiago Mem Inst Oswaldo Cruz Short Communication Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence. Instituto Oswaldo Cruz, Ministério da Saúde 2017-11 /pmc/articles/PMC5661903/ /pubmed/29091140 http://dx.doi.org/10.1590/0074-02760170105 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Villela, Anne Drumond
Rodrigues-Junior, Valnês S
Pinto, Antônio Frederico Michel
Falcão, Virgínia Carla de Almeida
Sánchez-Quitian, Zilpa Adriana
Eichler, Paula
Bizarro, Cristiano Valim
Basso, Luiz Augusto
Santos, Diógenes Santiago
Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
title Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
title_full Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
title_fullStr Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
title_full_unstemmed Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
title_short Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
title_sort construction of mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661903/
https://www.ncbi.nlm.nih.gov/pubmed/29091140
http://dx.doi.org/10.1590/0074-02760170105
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