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miRNA alteration is an important mechanism in sugarcane response to low-temperature environment
BACKGROUND: Cold is a major abiotic stress limiting the production of tropical and subtropical crops in new production areas. Sugarcane (Saccharum spp.) originates from the tropics but is cultivated primarily in the sub-tropics where it frequently encounters cold stress. Besides regulating plant gro...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661916/ https://www.ncbi.nlm.nih.gov/pubmed/29084515 http://dx.doi.org/10.1186/s12864-017-4231-3 |
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author | Yang, Yuting Zhang, Xu Su, Yachun Zou, Jiake Wang, Zhoutao Xu, Liping Que, Youxiong |
author_facet | Yang, Yuting Zhang, Xu Su, Yachun Zou, Jiake Wang, Zhoutao Xu, Liping Que, Youxiong |
author_sort | Yang, Yuting |
collection | PubMed |
description | BACKGROUND: Cold is a major abiotic stress limiting the production of tropical and subtropical crops in new production areas. Sugarcane (Saccharum spp.) originates from the tropics but is cultivated primarily in the sub-tropics where it frequently encounters cold stress. Besides regulating plant growth, miRNAs play an important role in environmental adaption. RESULTS: In this study, a total of 412 sugarcane miRNAs, including 261 known and 151 novel miRNAs, were obtained from 4 small RNA libraries through the Illumina sequencing method. Among them, 62 exhibited significant differential expression under cold stress, with 34 being upregulated and 28 being downregulated. The expression of 13 miRNAs and 12 corresponding targets was validated by RT-qPCR, with the majority being consistent with the sequencing data. GO and KEGG analysis indicated that these miRNAs were involved in stress-related biological pathways. To further investigate the involvement of these miRNAs in tolerance to abiotic stresses, sugarcane miR156 was selected for functional analysis. RT-qPCR revealed that miR156 levels increased in sugarcane during cold, salt and drought stress treatments. Nicotiana benthamiana plants transiently overexpressing miR156 exhibited better growth status, lower ROS levels, higher anthocyanin contents as well as the induction of some cold-responsive genes, suggesting its positive role in the plant cold stress response. CONCLUSIONS: This study provides a global view of the association of miRNA expression with the sugarcane response to cold stress. The findings have enriched the present miRNA resource and have made an attempt to verify the involvement of miR156 in plant response to cold stress. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-017-4231-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5661916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56619162017-11-01 miRNA alteration is an important mechanism in sugarcane response to low-temperature environment Yang, Yuting Zhang, Xu Su, Yachun Zou, Jiake Wang, Zhoutao Xu, Liping Que, Youxiong BMC Genomics Research Article BACKGROUND: Cold is a major abiotic stress limiting the production of tropical and subtropical crops in new production areas. Sugarcane (Saccharum spp.) originates from the tropics but is cultivated primarily in the sub-tropics where it frequently encounters cold stress. Besides regulating plant growth, miRNAs play an important role in environmental adaption. RESULTS: In this study, a total of 412 sugarcane miRNAs, including 261 known and 151 novel miRNAs, were obtained from 4 small RNA libraries through the Illumina sequencing method. Among them, 62 exhibited significant differential expression under cold stress, with 34 being upregulated and 28 being downregulated. The expression of 13 miRNAs and 12 corresponding targets was validated by RT-qPCR, with the majority being consistent with the sequencing data. GO and KEGG analysis indicated that these miRNAs were involved in stress-related biological pathways. To further investigate the involvement of these miRNAs in tolerance to abiotic stresses, sugarcane miR156 was selected for functional analysis. RT-qPCR revealed that miR156 levels increased in sugarcane during cold, salt and drought stress treatments. Nicotiana benthamiana plants transiently overexpressing miR156 exhibited better growth status, lower ROS levels, higher anthocyanin contents as well as the induction of some cold-responsive genes, suggesting its positive role in the plant cold stress response. CONCLUSIONS: This study provides a global view of the association of miRNA expression with the sugarcane response to cold stress. The findings have enriched the present miRNA resource and have made an attempt to verify the involvement of miR156 in plant response to cold stress. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-017-4231-3) contains supplementary material, which is available to authorized users. BioMed Central 2017-10-30 /pmc/articles/PMC5661916/ /pubmed/29084515 http://dx.doi.org/10.1186/s12864-017-4231-3 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Yang, Yuting Zhang, Xu Su, Yachun Zou, Jiake Wang, Zhoutao Xu, Liping Que, Youxiong miRNA alteration is an important mechanism in sugarcane response to low-temperature environment |
title | miRNA alteration is an important mechanism in sugarcane response to low-temperature environment |
title_full | miRNA alteration is an important mechanism in sugarcane response to low-temperature environment |
title_fullStr | miRNA alteration is an important mechanism in sugarcane response to low-temperature environment |
title_full_unstemmed | miRNA alteration is an important mechanism in sugarcane response to low-temperature environment |
title_short | miRNA alteration is an important mechanism in sugarcane response to low-temperature environment |
title_sort | mirna alteration is an important mechanism in sugarcane response to low-temperature environment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661916/ https://www.ncbi.nlm.nih.gov/pubmed/29084515 http://dx.doi.org/10.1186/s12864-017-4231-3 |
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