Cargando…

Evaluation of nutritional values, phenolic profile, aroma compounds and biological properties of Pittosporum tobira seeds

BACKGROUND: Plant essential oils and phenolic compounds are widely used for their medicinal properties. Thus, the aim of this study is to evaluate the nutritional values, the chemical composition, antioxidant activity and anti-hemolytic effects of Pittosporum tobira seeds. METHODS: The aroma compoun...

Descripción completa

Detalles Bibliográficos
Autores principales: Rjeibi, Ilhem, Ncib, Sana, Ben Saad, Anouar, Souid, Sami
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661929/
https://www.ncbi.nlm.nih.gov/pubmed/29084557
http://dx.doi.org/10.1186/s12944-017-0596-1
_version_ 1783274567229243392
author Rjeibi, Ilhem
Ncib, Sana
Ben Saad, Anouar
Souid, Sami
author_facet Rjeibi, Ilhem
Ncib, Sana
Ben Saad, Anouar
Souid, Sami
author_sort Rjeibi, Ilhem
collection PubMed
description BACKGROUND: Plant essential oils and phenolic compounds are widely used for their medicinal properties. Thus, the aim of this study is to evaluate the nutritional values, the chemical composition, antioxidant activity and anti-hemolytic effects of Pittosporum tobira seeds. METHODS: The aroma compounds were isolated using two methods (Headspace-solid phase microextraction (HS-SPME) and hydrodistillation (HD)) and analyzed by gas chromatography coupled with mass spectrometry (GC-MS). Bioactive phenolic compounds were identified by mean of high-performance liquid chromatography (HPLC-DAD). Reducing power, hydrogen peroxide (H(2)O(2)) scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays were used to investigate antioxidant activity. Anti-hemolytic activity was evaluated using H(2)O(2)-induced hemolysis of red blood cells (RBC). RESULTS: Oxygenated sesquiterpenes, sesquiterpene hydrocarbons and oxygenated monoterpenes were the most volatile fractions identified by HD and HS-SPME coupled to GC-MS but their quality and amount were quite different according to the extraction methodology. The main phenolic compounds identified by HPLC were caffeic acid, followed by cinnamic acid and gallic acid. P. tobira seeds essential oils showed significant antioxidant activity in DPPH (IC(50) value = 1.5 mg/mL), H(2)O(2) scavenging assay (IC(50) value = 159.43 μg/mL) and reducing power test (IC(50) value = 0.982 mg/mL) compared to methanolic extract. Moreover, the results revealed that the essential oil was able to protect RBC from hemolysis induced by H(2)O(2). However, the methanolic extract had no effect on H(2)O(2)-induced hemolysis of RBC as compared to the essential oil and the standard vitamin C. CONCLUSIONS: P. tobira may be used as a new natural source of antioxidant with therapeutic application in diseases caused by reactive oxygen species. GRAPHICAL ABSTRACT: [Image: see text] Phytochemical Characterization and Biological Evaluation of Pittosporum tobira seeds
format Online
Article
Text
id pubmed-5661929
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-56619292017-11-01 Evaluation of nutritional values, phenolic profile, aroma compounds and biological properties of Pittosporum tobira seeds Rjeibi, Ilhem Ncib, Sana Ben Saad, Anouar Souid, Sami Lipids Health Dis Research BACKGROUND: Plant essential oils and phenolic compounds are widely used for their medicinal properties. Thus, the aim of this study is to evaluate the nutritional values, the chemical composition, antioxidant activity and anti-hemolytic effects of Pittosporum tobira seeds. METHODS: The aroma compounds were isolated using two methods (Headspace-solid phase microextraction (HS-SPME) and hydrodistillation (HD)) and analyzed by gas chromatography coupled with mass spectrometry (GC-MS). Bioactive phenolic compounds were identified by mean of high-performance liquid chromatography (HPLC-DAD). Reducing power, hydrogen peroxide (H(2)O(2)) scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays were used to investigate antioxidant activity. Anti-hemolytic activity was evaluated using H(2)O(2)-induced hemolysis of red blood cells (RBC). RESULTS: Oxygenated sesquiterpenes, sesquiterpene hydrocarbons and oxygenated monoterpenes were the most volatile fractions identified by HD and HS-SPME coupled to GC-MS but their quality and amount were quite different according to the extraction methodology. The main phenolic compounds identified by HPLC were caffeic acid, followed by cinnamic acid and gallic acid. P. tobira seeds essential oils showed significant antioxidant activity in DPPH (IC(50) value = 1.5 mg/mL), H(2)O(2) scavenging assay (IC(50) value = 159.43 μg/mL) and reducing power test (IC(50) value = 0.982 mg/mL) compared to methanolic extract. Moreover, the results revealed that the essential oil was able to protect RBC from hemolysis induced by H(2)O(2). However, the methanolic extract had no effect on H(2)O(2)-induced hemolysis of RBC as compared to the essential oil and the standard vitamin C. CONCLUSIONS: P. tobira may be used as a new natural source of antioxidant with therapeutic application in diseases caused by reactive oxygen species. GRAPHICAL ABSTRACT: [Image: see text] Phytochemical Characterization and Biological Evaluation of Pittosporum tobira seeds BioMed Central 2017-10-30 /pmc/articles/PMC5661929/ /pubmed/29084557 http://dx.doi.org/10.1186/s12944-017-0596-1 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Rjeibi, Ilhem
Ncib, Sana
Ben Saad, Anouar
Souid, Sami
Evaluation of nutritional values, phenolic profile, aroma compounds and biological properties of Pittosporum tobira seeds
title Evaluation of nutritional values, phenolic profile, aroma compounds and biological properties of Pittosporum tobira seeds
title_full Evaluation of nutritional values, phenolic profile, aroma compounds and biological properties of Pittosporum tobira seeds
title_fullStr Evaluation of nutritional values, phenolic profile, aroma compounds and biological properties of Pittosporum tobira seeds
title_full_unstemmed Evaluation of nutritional values, phenolic profile, aroma compounds and biological properties of Pittosporum tobira seeds
title_short Evaluation of nutritional values, phenolic profile, aroma compounds and biological properties of Pittosporum tobira seeds
title_sort evaluation of nutritional values, phenolic profile, aroma compounds and biological properties of pittosporum tobira seeds
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661929/
https://www.ncbi.nlm.nih.gov/pubmed/29084557
http://dx.doi.org/10.1186/s12944-017-0596-1
work_keys_str_mv AT rjeibiilhem evaluationofnutritionalvaluesphenolicprofilearomacompoundsandbiologicalpropertiesofpittosporumtobiraseeds
AT ncibsana evaluationofnutritionalvaluesphenolicprofilearomacompoundsandbiologicalpropertiesofpittosporumtobiraseeds
AT bensaadanouar evaluationofnutritionalvaluesphenolicprofilearomacompoundsandbiologicalpropertiesofpittosporumtobiraseeds
AT souidsami evaluationofnutritionalvaluesphenolicprofilearomacompoundsandbiologicalpropertiesofpittosporumtobiraseeds