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Single Molecule Trapping and Sensing Using Dual Nanopores Separated by a Zeptoliter Nanobridge
[Image: see text] There is a growing realization, especially within the diagnostic and therapeutic community, that the amount of information enclosed in a single molecule can not only enable a better understanding of biophysical pathways, but also offer exceptional value for early stage biomarker de...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5662926/ https://www.ncbi.nlm.nih.gov/pubmed/28862004 http://dx.doi.org/10.1021/acs.nanolett.7b03196 |
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author | Cadinu, Paolo Paulose Nadappuram, Binoy Lee, Dominic J. Sze, Jasmine Y. Y. Campolo, Giulia Zhang, Yanjun Shevchuk, Andrew Ladame, Sylvain Albrecht, Tim Korchev, Yuri Ivanov, Aleksandar P. Edel, Joshua B. |
author_facet | Cadinu, Paolo Paulose Nadappuram, Binoy Lee, Dominic J. Sze, Jasmine Y. Y. Campolo, Giulia Zhang, Yanjun Shevchuk, Andrew Ladame, Sylvain Albrecht, Tim Korchev, Yuri Ivanov, Aleksandar P. Edel, Joshua B. |
author_sort | Cadinu, Paolo |
collection | PubMed |
description | [Image: see text] There is a growing realization, especially within the diagnostic and therapeutic community, that the amount of information enclosed in a single molecule can not only enable a better understanding of biophysical pathways, but also offer exceptional value for early stage biomarker detection of disease onset. To this end, numerous single molecule strategies have been proposed, and in terms of label-free routes, nanopore sensing has emerged as one of the most promising methods. However, being able to finely control molecular transport in terms of transport rate, resolution, and signal-to-noise ratio (SNR) is essential to take full advantage of the technology benefits. Here we propose a novel solution to these challenges based on a method that allows biomolecules to be individually confined into a zeptoliter nanoscale droplet bridging two adjacent nanopores (nanobridge) with a 20 nm separation. Molecules that undergo confinement in the nanobridge are slowed down by up to 3 orders of magnitude compared to conventional nanopores. This leads to a dramatic improvement in the SNR, resolution, sensitivity, and limit of detection. The strategy implemented is universal and as highlighted in this manuscript can be used for the detection of dsDNA, RNA, ssDNA, and proteins. |
format | Online Article Text |
id | pubmed-5662926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-56629262017-11-01 Single Molecule Trapping and Sensing Using Dual Nanopores Separated by a Zeptoliter Nanobridge Cadinu, Paolo Paulose Nadappuram, Binoy Lee, Dominic J. Sze, Jasmine Y. Y. Campolo, Giulia Zhang, Yanjun Shevchuk, Andrew Ladame, Sylvain Albrecht, Tim Korchev, Yuri Ivanov, Aleksandar P. Edel, Joshua B. Nano Lett [Image: see text] There is a growing realization, especially within the diagnostic and therapeutic community, that the amount of information enclosed in a single molecule can not only enable a better understanding of biophysical pathways, but also offer exceptional value for early stage biomarker detection of disease onset. To this end, numerous single molecule strategies have been proposed, and in terms of label-free routes, nanopore sensing has emerged as one of the most promising methods. However, being able to finely control molecular transport in terms of transport rate, resolution, and signal-to-noise ratio (SNR) is essential to take full advantage of the technology benefits. Here we propose a novel solution to these challenges based on a method that allows biomolecules to be individually confined into a zeptoliter nanoscale droplet bridging two adjacent nanopores (nanobridge) with a 20 nm separation. Molecules that undergo confinement in the nanobridge are slowed down by up to 3 orders of magnitude compared to conventional nanopores. This leads to a dramatic improvement in the SNR, resolution, sensitivity, and limit of detection. The strategy implemented is universal and as highlighted in this manuscript can be used for the detection of dsDNA, RNA, ssDNA, and proteins. American Chemical Society 2017-09-01 2017-10-11 /pmc/articles/PMC5662926/ /pubmed/28862004 http://dx.doi.org/10.1021/acs.nanolett.7b03196 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Cadinu, Paolo Paulose Nadappuram, Binoy Lee, Dominic J. Sze, Jasmine Y. Y. Campolo, Giulia Zhang, Yanjun Shevchuk, Andrew Ladame, Sylvain Albrecht, Tim Korchev, Yuri Ivanov, Aleksandar P. Edel, Joshua B. Single Molecule Trapping and Sensing Using Dual Nanopores Separated by a Zeptoliter Nanobridge |
title | Single Molecule Trapping and Sensing Using Dual Nanopores
Separated by a Zeptoliter Nanobridge |
title_full | Single Molecule Trapping and Sensing Using Dual Nanopores
Separated by a Zeptoliter Nanobridge |
title_fullStr | Single Molecule Trapping and Sensing Using Dual Nanopores
Separated by a Zeptoliter Nanobridge |
title_full_unstemmed | Single Molecule Trapping and Sensing Using Dual Nanopores
Separated by a Zeptoliter Nanobridge |
title_short | Single Molecule Trapping and Sensing Using Dual Nanopores
Separated by a Zeptoliter Nanobridge |
title_sort | single molecule trapping and sensing using dual nanopores
separated by a zeptoliter nanobridge |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5662926/ https://www.ncbi.nlm.nih.gov/pubmed/28862004 http://dx.doi.org/10.1021/acs.nanolett.7b03196 |
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