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Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins
The aim of the current study was to compare the antitumor efficiency of Phellinus igniarius polysaccharides (PIP) from six different origins and preliminarily investigate its potential mechanisms. PIP was extracted using the microwave extraction method. The corresponding in vivo antitumor efficacy w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663028/ https://www.ncbi.nlm.nih.gov/pubmed/29109758 http://dx.doi.org/10.3892/etm.2017.5191 |
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author | Gao, Wenwen Wang, Wangdi Sun, Wenjian Wang, Mingfang Zhang, Na Yu, Shuwen |
author_facet | Gao, Wenwen Wang, Wangdi Sun, Wenjian Wang, Mingfang Zhang, Na Yu, Shuwen |
author_sort | Gao, Wenwen |
collection | PubMed |
description | The aim of the current study was to compare the antitumor efficiency of Phellinus igniarius polysaccharides (PIP) from six different origins and preliminarily investigate its potential mechanisms. PIP was extracted using the microwave extraction method. The corresponding in vivo antitumor efficacy was assessed in Kunming mice bearing H22 tumors and Gansu PIP (GPIP) was identified to have a significantly higher antitumor efficacy compared with the control group (P<0.05), while no significant difference was observed following treatment with PIP from different origins (P>0.05). The spleen index of the GPIP group significantly increased compared with the saline and CTX groups (P<0.01). The in vitro MTT assay of GPIP on HepG2 cells indicated that GPIP had no direct cytotoxicity. The serum immune cytokines of interleukin-2, interleukin-12 and interferon-γ were assessed using the ELISA method. The concentration of all three serum cytokines significantly increased compared with saline and CTX groups (P<0.01) indicating that activating the immune system may be a potential antitumor mechanism. These results demonstrated that GPIP has great potential as a natural antitumor agent with immunomodulatory activity. |
format | Online Article Text |
id | pubmed-5663028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-56630282017-11-06 Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins Gao, Wenwen Wang, Wangdi Sun, Wenjian Wang, Mingfang Zhang, Na Yu, Shuwen Exp Ther Med Articles The aim of the current study was to compare the antitumor efficiency of Phellinus igniarius polysaccharides (PIP) from six different origins and preliminarily investigate its potential mechanisms. PIP was extracted using the microwave extraction method. The corresponding in vivo antitumor efficacy was assessed in Kunming mice bearing H22 tumors and Gansu PIP (GPIP) was identified to have a significantly higher antitumor efficacy compared with the control group (P<0.05), while no significant difference was observed following treatment with PIP from different origins (P>0.05). The spleen index of the GPIP group significantly increased compared with the saline and CTX groups (P<0.01). The in vitro MTT assay of GPIP on HepG2 cells indicated that GPIP had no direct cytotoxicity. The serum immune cytokines of interleukin-2, interleukin-12 and interferon-γ were assessed using the ELISA method. The concentration of all three serum cytokines significantly increased compared with saline and CTX groups (P<0.01) indicating that activating the immune system may be a potential antitumor mechanism. These results demonstrated that GPIP has great potential as a natural antitumor agent with immunomodulatory activity. D.A. Spandidos 2017-11 2017-09-22 /pmc/articles/PMC5663028/ /pubmed/29109758 http://dx.doi.org/10.3892/etm.2017.5191 Text en Copyright: © Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Gao, Wenwen Wang, Wangdi Sun, Wenjian Wang, Mingfang Zhang, Na Yu, Shuwen Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins |
title | Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins |
title_full | Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins |
title_fullStr | Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins |
title_full_unstemmed | Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins |
title_short | Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins |
title_sort | antitumor and immunomodulating activities of six phellinus igniarius polysaccharides of different origins |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663028/ https://www.ncbi.nlm.nih.gov/pubmed/29109758 http://dx.doi.org/10.3892/etm.2017.5191 |
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