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Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins

The aim of the current study was to compare the antitumor efficiency of Phellinus igniarius polysaccharides (PIP) from six different origins and preliminarily investigate its potential mechanisms. PIP was extracted using the microwave extraction method. The corresponding in vivo antitumor efficacy w...

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Detalles Bibliográficos
Autores principales: Gao, Wenwen, Wang, Wangdi, Sun, Wenjian, Wang, Mingfang, Zhang, Na, Yu, Shuwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663028/
https://www.ncbi.nlm.nih.gov/pubmed/29109758
http://dx.doi.org/10.3892/etm.2017.5191
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author Gao, Wenwen
Wang, Wangdi
Sun, Wenjian
Wang, Mingfang
Zhang, Na
Yu, Shuwen
author_facet Gao, Wenwen
Wang, Wangdi
Sun, Wenjian
Wang, Mingfang
Zhang, Na
Yu, Shuwen
author_sort Gao, Wenwen
collection PubMed
description The aim of the current study was to compare the antitumor efficiency of Phellinus igniarius polysaccharides (PIP) from six different origins and preliminarily investigate its potential mechanisms. PIP was extracted using the microwave extraction method. The corresponding in vivo antitumor efficacy was assessed in Kunming mice bearing H22 tumors and Gansu PIP (GPIP) was identified to have a significantly higher antitumor efficacy compared with the control group (P<0.05), while no significant difference was observed following treatment with PIP from different origins (P>0.05). The spleen index of the GPIP group significantly increased compared with the saline and CTX groups (P<0.01). The in vitro MTT assay of GPIP on HepG2 cells indicated that GPIP had no direct cytotoxicity. The serum immune cytokines of interleukin-2, interleukin-12 and interferon-γ were assessed using the ELISA method. The concentration of all three serum cytokines significantly increased compared with saline and CTX groups (P<0.01) indicating that activating the immune system may be a potential antitumor mechanism. These results demonstrated that GPIP has great potential as a natural antitumor agent with immunomodulatory activity.
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spelling pubmed-56630282017-11-06 Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins Gao, Wenwen Wang, Wangdi Sun, Wenjian Wang, Mingfang Zhang, Na Yu, Shuwen Exp Ther Med Articles The aim of the current study was to compare the antitumor efficiency of Phellinus igniarius polysaccharides (PIP) from six different origins and preliminarily investigate its potential mechanisms. PIP was extracted using the microwave extraction method. The corresponding in vivo antitumor efficacy was assessed in Kunming mice bearing H22 tumors and Gansu PIP (GPIP) was identified to have a significantly higher antitumor efficacy compared with the control group (P<0.05), while no significant difference was observed following treatment with PIP from different origins (P>0.05). The spleen index of the GPIP group significantly increased compared with the saline and CTX groups (P<0.01). The in vitro MTT assay of GPIP on HepG2 cells indicated that GPIP had no direct cytotoxicity. The serum immune cytokines of interleukin-2, interleukin-12 and interferon-γ were assessed using the ELISA method. The concentration of all three serum cytokines significantly increased compared with saline and CTX groups (P<0.01) indicating that activating the immune system may be a potential antitumor mechanism. These results demonstrated that GPIP has great potential as a natural antitumor agent with immunomodulatory activity. D.A. Spandidos 2017-11 2017-09-22 /pmc/articles/PMC5663028/ /pubmed/29109758 http://dx.doi.org/10.3892/etm.2017.5191 Text en Copyright: © Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Gao, Wenwen
Wang, Wangdi
Sun, Wenjian
Wang, Mingfang
Zhang, Na
Yu, Shuwen
Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins
title Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins
title_full Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins
title_fullStr Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins
title_full_unstemmed Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins
title_short Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins
title_sort antitumor and immunomodulating activities of six phellinus igniarius polysaccharides of different origins
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663028/
https://www.ncbi.nlm.nih.gov/pubmed/29109758
http://dx.doi.org/10.3892/etm.2017.5191
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