The protective effects of carboxyhemoglobin during the resuscitation from hemorrhagic shock in rats

AIM: This study was aimed to explore the effects of carboxyhemoglobin on reperfusion injury in hemorrhagic shock, as well as its action time and related mechanisms. RESULTS: CO-RBC group showed milder oxidative injury than O2-RBC group. CO reperfusion did not show advantages in functions of kidney and lung during resuscitation. The level of Bax was decreased in CO-RBC group, especially in early CO-RBC group. Moreover, the autophay-related gene Beclin-1 was down-regulated in CO-RBC and early CO-RBC groups. The inflammation was severer in CO-RBC resuscitation group. MATERIALS AND METHODS: The hemorrhagic shock model rats were randomly divided into: the hemorrhagic shock group (n = 6); the O2-red blood cells (O(2)-RBC) group (n = 6), perfused with O(2)-RBC 1 h after ischemia; CO-RBC group (n = 12), perfused with CO-RBC 1 h after ischemia; and early CO-RBC group (n = 12), perfused with CO-RBC 30 min after ischemia. The reperfusion injuries were evaluated through anti-reactive oxygen species (ROS), inflammatory action, organ function, cell apoptosis and autophagy. CONCLUSIONS: Carboxyhemoglobin not only relieves the oxidative injury and inhibites apoptosis and autophagy, but also aggravates inflammatory reactions during reperfusion. The action time of carboxyhemoglobin may be an influencing factor for reperfusion outcomes.