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Genome-wide analysis and functional prediction of long non-coding RNAs in mouse uterus during the implantation window
Establishment of the receptive uterus is a crucial step for embryo implantation. In this study, the expression profiles and characterization of long non-coding RNAs (lncRNAs) in pregnant mouse uteri on day 4, day 5 at implantation sites and inter-implantation sites were conducted using RNA-seq. A to...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663602/ https://www.ncbi.nlm.nih.gov/pubmed/29137430 http://dx.doi.org/10.18632/oncotarget.21031 |
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author | Wang, Qi Wang, Nan Cai, Rui Zhao, Fan Xiong, Yongjie Li, Xiao Wang, Aihua Lin, Pengfei Jin, Yaping |
author_facet | Wang, Qi Wang, Nan Cai, Rui Zhao, Fan Xiong, Yongjie Li, Xiao Wang, Aihua Lin, Pengfei Jin, Yaping |
author_sort | Wang, Qi |
collection | PubMed |
description | Establishment of the receptive uterus is a crucial step for embryo implantation. In this study, the expression profiles and characterization of long non-coding RNAs (lncRNAs) in pregnant mouse uteri on day 4, day 5 at implantation sites and inter-implantation sites were conducted using RNA-seq. A total of 7,764 putative lncRNA transcripts were identified, including 6,179 known lncRNA transcripts and 1,585 novel lncRNA transcripts. Bioinformatics analysis of the cis and trans lncRNA targets showed that the differentially expressed lncRNAs were mainly involved in tissue remodelling, immune response and metabolism-related processes, indicating that lncRNAs could be involved in the regulation of embryo implantation. We also discovered that differentially expressed lncRNAs might regulate multiple signalling pathways that play an important role in the regulation of embryo implantation. In addition, nine known lncRNAs and four novel lncRNAs were randomly selected and validated by qRT-PCR. The expression of Tug1, Neat1, Gas5, Malat1, H19 and Rmst were significantly regulated in the mouse uterus during the implantation window. Our results are the first to systematically identify lncRNAs in the mouse uterus and provide a catalogue of lncRNAs for further understanding their functions in pregnant mouse uteri during the implantation window. |
format | Online Article Text |
id | pubmed-5663602 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56636022017-11-13 Genome-wide analysis and functional prediction of long non-coding RNAs in mouse uterus during the implantation window Wang, Qi Wang, Nan Cai, Rui Zhao, Fan Xiong, Yongjie Li, Xiao Wang, Aihua Lin, Pengfei Jin, Yaping Oncotarget Research Paper Establishment of the receptive uterus is a crucial step for embryo implantation. In this study, the expression profiles and characterization of long non-coding RNAs (lncRNAs) in pregnant mouse uteri on day 4, day 5 at implantation sites and inter-implantation sites were conducted using RNA-seq. A total of 7,764 putative lncRNA transcripts were identified, including 6,179 known lncRNA transcripts and 1,585 novel lncRNA transcripts. Bioinformatics analysis of the cis and trans lncRNA targets showed that the differentially expressed lncRNAs were mainly involved in tissue remodelling, immune response and metabolism-related processes, indicating that lncRNAs could be involved in the regulation of embryo implantation. We also discovered that differentially expressed lncRNAs might regulate multiple signalling pathways that play an important role in the regulation of embryo implantation. In addition, nine known lncRNAs and four novel lncRNAs were randomly selected and validated by qRT-PCR. The expression of Tug1, Neat1, Gas5, Malat1, H19 and Rmst were significantly regulated in the mouse uterus during the implantation window. Our results are the first to systematically identify lncRNAs in the mouse uterus and provide a catalogue of lncRNAs for further understanding their functions in pregnant mouse uteri during the implantation window. Impact Journals LLC 2017-09-16 /pmc/articles/PMC5663602/ /pubmed/29137430 http://dx.doi.org/10.18632/oncotarget.21031 Text en Copyright: © 2017 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wang, Qi Wang, Nan Cai, Rui Zhao, Fan Xiong, Yongjie Li, Xiao Wang, Aihua Lin, Pengfei Jin, Yaping Genome-wide analysis and functional prediction of long non-coding RNAs in mouse uterus during the implantation window |
title | Genome-wide analysis and functional prediction of long non-coding RNAs in mouse uterus during the implantation window |
title_full | Genome-wide analysis and functional prediction of long non-coding RNAs in mouse uterus during the implantation window |
title_fullStr | Genome-wide analysis and functional prediction of long non-coding RNAs in mouse uterus during the implantation window |
title_full_unstemmed | Genome-wide analysis and functional prediction of long non-coding RNAs in mouse uterus during the implantation window |
title_short | Genome-wide analysis and functional prediction of long non-coding RNAs in mouse uterus during the implantation window |
title_sort | genome-wide analysis and functional prediction of long non-coding rnas in mouse uterus during the implantation window |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663602/ https://www.ncbi.nlm.nih.gov/pubmed/29137430 http://dx.doi.org/10.18632/oncotarget.21031 |
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