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A Multiplex SYBR Green Real-Time PCR Assay for the Detection of Three Colistin Resistance Genes from Cultured Bacteria, Feces, and Environment Samples

The aim of the study was to develop a multiplex assay for rapid detection of mcr-1, mcr-2, and mcr-3, a group of genes of conferring resistance to colistin mediated by plasmid in Enterobacteriaceae. A SYBR Green based real-time PCR assay has been designed to detect the mcr genes, and applied to cult...

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Detalles Bibliográficos
Autores principales:	Li, Jiyun, Shi, Xiaomin, Yin, Wenjuan, Wang, Yang, Shen, Zhangqi, Ding, Shuangyang, Wang, Shaolin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2017
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663727/ https://www.ncbi.nlm.nih.gov/pubmed/29163387 http://dx.doi.org/10.3389/fmicb.2017.02078

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