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Comparison of nasal cytokine profiles of human metapneumovirus and respiratory syncytial virus

BACKGROUND: Human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) share some epidemiological and clinical characteristics; however, few studies have examined the mechanisms by which these viruses induce airway inflammation. OBJECTIVE: This study was undertaken to compare cytokine profil...

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Autores principales: Park, Jin-Sung, Kim, Young-Ho, Kwon, Eunmi, Callaway, Zak, Fujisawa, Takao, Kim, Chang-Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asia Pacific Association of Allergy, Asthma and Clinical Immunology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663748/
https://www.ncbi.nlm.nih.gov/pubmed/29094018
http://dx.doi.org/10.5415/apallergy.2017.7.4.206
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author Park, Jin-Sung
Kim, Young-Ho
Kwon, Eunmi
Callaway, Zak
Fujisawa, Takao
Kim, Chang-Keun
author_facet Park, Jin-Sung
Kim, Young-Ho
Kwon, Eunmi
Callaway, Zak
Fujisawa, Takao
Kim, Chang-Keun
author_sort Park, Jin-Sung
collection PubMed
description BACKGROUND: Human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) share some epidemiological and clinical characteristics; however, few studies have examined the mechanisms by which these viruses induce airway inflammation. OBJECTIVE: This study was undertaken to compare cytokine profiles in hMPV and RSV patients to investigate possible differences in inflammatory pathways. METHODS: Nasopharyngeal aspirate specimens were collected from 1,008 pediatric patients hospitalized for acute lower respiratory tract infection with wheezing and 20 normal healthy controls. Patients were tested for 7 common respiratory viruses then divided into hMPV (n = 35) and RSV groups (n = 67). T helper (Th) 1 (interferon [IFN]-γ), Th2 (interleukin [IL]-4, eotaxin) and Th17 (IL-1β, IL-6) cytokine profiles were analyzed in the 3 groups. RESULTS: IFN-γ and IL-2 levels were significantly increased in the hMPV and RSV groups compared to the control group (p < 0.0001 and p < 0.0001, respectively). IL-4 levels were significantly higher in the RSV group compared to the hMPV and control groups (p = 0.0003 and p < 0.0001, respectively). Eotaxin levels showed a tendency to be higher in the RSV group compared to the hMPV group (p = 0.0580), and significantly higher compared to the control group (p < 0.0001). IL-1β levels were significantly higher in the hMPV compared to the RSV group (p < 0.0001), and IL-6 levels were significantly higher in the hMPV group compared to the control group (p < 0.0001). CONCLUSION: Our results suggest that hMPV and RSV have different inflammatory mechanisms. hMPV induces airway inflammation by the Th17 pathway through release of IL-1β and IL-6, whereas RSV acts through the Th2 pathway.
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spelling pubmed-56637482017-11-01 Comparison of nasal cytokine profiles of human metapneumovirus and respiratory syncytial virus Park, Jin-Sung Kim, Young-Ho Kwon, Eunmi Callaway, Zak Fujisawa, Takao Kim, Chang-Keun Asia Pac Allergy Original Article BACKGROUND: Human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) share some epidemiological and clinical characteristics; however, few studies have examined the mechanisms by which these viruses induce airway inflammation. OBJECTIVE: This study was undertaken to compare cytokine profiles in hMPV and RSV patients to investigate possible differences in inflammatory pathways. METHODS: Nasopharyngeal aspirate specimens were collected from 1,008 pediatric patients hospitalized for acute lower respiratory tract infection with wheezing and 20 normal healthy controls. Patients were tested for 7 common respiratory viruses then divided into hMPV (n = 35) and RSV groups (n = 67). T helper (Th) 1 (interferon [IFN]-γ), Th2 (interleukin [IL]-4, eotaxin) and Th17 (IL-1β, IL-6) cytokine profiles were analyzed in the 3 groups. RESULTS: IFN-γ and IL-2 levels were significantly increased in the hMPV and RSV groups compared to the control group (p < 0.0001 and p < 0.0001, respectively). IL-4 levels were significantly higher in the RSV group compared to the hMPV and control groups (p = 0.0003 and p < 0.0001, respectively). Eotaxin levels showed a tendency to be higher in the RSV group compared to the hMPV group (p = 0.0580), and significantly higher compared to the control group (p < 0.0001). IL-1β levels were significantly higher in the hMPV compared to the RSV group (p < 0.0001), and IL-6 levels were significantly higher in the hMPV group compared to the control group (p < 0.0001). CONCLUSION: Our results suggest that hMPV and RSV have different inflammatory mechanisms. hMPV induces airway inflammation by the Th17 pathway through release of IL-1β and IL-6, whereas RSV acts through the Th2 pathway. Asia Pacific Association of Allergy, Asthma and Clinical Immunology 2017-10 2017-10-19 /pmc/articles/PMC5663748/ /pubmed/29094018 http://dx.doi.org/10.5415/apallergy.2017.7.4.206 Text en Copyright © 2017. Asia Pacific Association of Allergy, Asthma and Clinical Immunology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Park, Jin-Sung
Kim, Young-Ho
Kwon, Eunmi
Callaway, Zak
Fujisawa, Takao
Kim, Chang-Keun
Comparison of nasal cytokine profiles of human metapneumovirus and respiratory syncytial virus
title Comparison of nasal cytokine profiles of human metapneumovirus and respiratory syncytial virus
title_full Comparison of nasal cytokine profiles of human metapneumovirus and respiratory syncytial virus
title_fullStr Comparison of nasal cytokine profiles of human metapneumovirus and respiratory syncytial virus
title_full_unstemmed Comparison of nasal cytokine profiles of human metapneumovirus and respiratory syncytial virus
title_short Comparison of nasal cytokine profiles of human metapneumovirus and respiratory syncytial virus
title_sort comparison of nasal cytokine profiles of human metapneumovirus and respiratory syncytial virus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663748/
https://www.ncbi.nlm.nih.gov/pubmed/29094018
http://dx.doi.org/10.5415/apallergy.2017.7.4.206
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