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Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale
Dendrobium officinale is an extremely valuable orchid used in traditional Chinese medicine, so sought after that it has a higher market value than gold. Although the expression profiles of some genes involved in the polysaccharide synthesis have previously been investigated, little research has been...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664107/ https://www.ncbi.nlm.nih.gov/pubmed/28981454 http://dx.doi.org/10.3390/genes8100257 |
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author | He, Liu Fu, Shuhua Xu, Zhichao Yan, Jun Xu, Jiang Zhou, Hong Zhou, Jianguo Chen, Xinlian Li, Ying Au, Kin Fai Yao, Hui |
author_facet | He, Liu Fu, Shuhua Xu, Zhichao Yan, Jun Xu, Jiang Zhou, Hong Zhou, Jianguo Chen, Xinlian Li, Ying Au, Kin Fai Yao, Hui |
author_sort | He, Liu |
collection | PubMed |
description | Dendrobium officinale is an extremely valuable orchid used in traditional Chinese medicine, so sought after that it has a higher market value than gold. Although the expression profiles of some genes involved in the polysaccharide synthesis have previously been investigated, little research has been carried out on their alternatively spliced isoforms in D. officinale. In addition, information regarding the translocation of sugars from leaves to stems in D. officinale also remains limited. We analyzed the polysaccharide content of D. officinale leaves and stems, and completed in-depth transcriptome sequencing of these two diverse tissue types using second-generation sequencing (SGS) and single-molecule real-time (SMRT) sequencing technology. The results of this study yielded a digital inventory of gene and mRNA isoform expressions. A comparative analysis of both transcriptomes uncovered a total of 1414 differentially expressed genes, including 844 that were up-regulated and 570 that were down-regulated in stems. Of these genes, one sugars will eventually be exported transporter (SWEET) and one sucrose transporter (SUT) are expressed to a greater extent in D. officinale stems than in leaves. Two glycosyltransferase (GT) and four cellulose synthase (Ces) genes undergo a distinct degree of alternative splicing. In the stems, the content of polysaccharides is twice as much as that in the leaves. The differentially expressed GT and transcription factor (TF) genes will be the focus of further study. The genes DoSWEET4 and DoSUT1 are significantly expressed in the stem, and are likely to be involved in sugar loading in the phloem. |
format | Online Article Text |
id | pubmed-5664107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56641072017-11-06 Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale He, Liu Fu, Shuhua Xu, Zhichao Yan, Jun Xu, Jiang Zhou, Hong Zhou, Jianguo Chen, Xinlian Li, Ying Au, Kin Fai Yao, Hui Genes (Basel) Article Dendrobium officinale is an extremely valuable orchid used in traditional Chinese medicine, so sought after that it has a higher market value than gold. Although the expression profiles of some genes involved in the polysaccharide synthesis have previously been investigated, little research has been carried out on their alternatively spliced isoforms in D. officinale. In addition, information regarding the translocation of sugars from leaves to stems in D. officinale also remains limited. We analyzed the polysaccharide content of D. officinale leaves and stems, and completed in-depth transcriptome sequencing of these two diverse tissue types using second-generation sequencing (SGS) and single-molecule real-time (SMRT) sequencing technology. The results of this study yielded a digital inventory of gene and mRNA isoform expressions. A comparative analysis of both transcriptomes uncovered a total of 1414 differentially expressed genes, including 844 that were up-regulated and 570 that were down-regulated in stems. Of these genes, one sugars will eventually be exported transporter (SWEET) and one sucrose transporter (SUT) are expressed to a greater extent in D. officinale stems than in leaves. Two glycosyltransferase (GT) and four cellulose synthase (Ces) genes undergo a distinct degree of alternative splicing. In the stems, the content of polysaccharides is twice as much as that in the leaves. The differentially expressed GT and transcription factor (TF) genes will be the focus of further study. The genes DoSWEET4 and DoSUT1 are significantly expressed in the stem, and are likely to be involved in sugar loading in the phloem. MDPI 2017-10-05 /pmc/articles/PMC5664107/ /pubmed/28981454 http://dx.doi.org/10.3390/genes8100257 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article He, Liu Fu, Shuhua Xu, Zhichao Yan, Jun Xu, Jiang Zhou, Hong Zhou, Jianguo Chen, Xinlian Li, Ying Au, Kin Fai Yao, Hui Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale |
title | Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale |
title_full | Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale |
title_fullStr | Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale |
title_full_unstemmed | Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale |
title_short | Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale |
title_sort | hybrid sequencing of full-length cdna transcripts of stems and leaves in dendrobium officinale |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664107/ https://www.ncbi.nlm.nih.gov/pubmed/28981454 http://dx.doi.org/10.3390/genes8100257 |
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