Cargando…

Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale

Dendrobium officinale is an extremely valuable orchid used in traditional Chinese medicine, so sought after that it has a higher market value than gold. Although the expression profiles of some genes involved in the polysaccharide synthesis have previously been investigated, little research has been...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Liu, Fu, Shuhua, Xu, Zhichao, Yan, Jun, Xu, Jiang, Zhou, Hong, Zhou, Jianguo, Chen, Xinlian, Li, Ying, Au, Kin Fai, Yao, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664107/
https://www.ncbi.nlm.nih.gov/pubmed/28981454
http://dx.doi.org/10.3390/genes8100257
_version_ 1783274940169977856
author He, Liu
Fu, Shuhua
Xu, Zhichao
Yan, Jun
Xu, Jiang
Zhou, Hong
Zhou, Jianguo
Chen, Xinlian
Li, Ying
Au, Kin Fai
Yao, Hui
author_facet He, Liu
Fu, Shuhua
Xu, Zhichao
Yan, Jun
Xu, Jiang
Zhou, Hong
Zhou, Jianguo
Chen, Xinlian
Li, Ying
Au, Kin Fai
Yao, Hui
author_sort He, Liu
collection PubMed
description Dendrobium officinale is an extremely valuable orchid used in traditional Chinese medicine, so sought after that it has a higher market value than gold. Although the expression profiles of some genes involved in the polysaccharide synthesis have previously been investigated, little research has been carried out on their alternatively spliced isoforms in D. officinale. In addition, information regarding the translocation of sugars from leaves to stems in D. officinale also remains limited. We analyzed the polysaccharide content of D. officinale leaves and stems, and completed in-depth transcriptome sequencing of these two diverse tissue types using second-generation sequencing (SGS) and single-molecule real-time (SMRT) sequencing technology. The results of this study yielded a digital inventory of gene and mRNA isoform expressions. A comparative analysis of both transcriptomes uncovered a total of 1414 differentially expressed genes, including 844 that were up-regulated and 570 that were down-regulated in stems. Of these genes, one sugars will eventually be exported transporter (SWEET) and one sucrose transporter (SUT) are expressed to a greater extent in D. officinale stems than in leaves. Two glycosyltransferase (GT) and four cellulose synthase (Ces) genes undergo a distinct degree of alternative splicing. In the stems, the content of polysaccharides is twice as much as that in the leaves. The differentially expressed GT and transcription factor (TF) genes will be the focus of further study. The genes DoSWEET4 and DoSUT1 are significantly expressed in the stem, and are likely to be involved in sugar loading in the phloem.
format Online
Article
Text
id pubmed-5664107
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-56641072017-11-06 Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale He, Liu Fu, Shuhua Xu, Zhichao Yan, Jun Xu, Jiang Zhou, Hong Zhou, Jianguo Chen, Xinlian Li, Ying Au, Kin Fai Yao, Hui Genes (Basel) Article Dendrobium officinale is an extremely valuable orchid used in traditional Chinese medicine, so sought after that it has a higher market value than gold. Although the expression profiles of some genes involved in the polysaccharide synthesis have previously been investigated, little research has been carried out on their alternatively spliced isoforms in D. officinale. In addition, information regarding the translocation of sugars from leaves to stems in D. officinale also remains limited. We analyzed the polysaccharide content of D. officinale leaves and stems, and completed in-depth transcriptome sequencing of these two diverse tissue types using second-generation sequencing (SGS) and single-molecule real-time (SMRT) sequencing technology. The results of this study yielded a digital inventory of gene and mRNA isoform expressions. A comparative analysis of both transcriptomes uncovered a total of 1414 differentially expressed genes, including 844 that were up-regulated and 570 that were down-regulated in stems. Of these genes, one sugars will eventually be exported transporter (SWEET) and one sucrose transporter (SUT) are expressed to a greater extent in D. officinale stems than in leaves. Two glycosyltransferase (GT) and four cellulose synthase (Ces) genes undergo a distinct degree of alternative splicing. In the stems, the content of polysaccharides is twice as much as that in the leaves. The differentially expressed GT and transcription factor (TF) genes will be the focus of further study. The genes DoSWEET4 and DoSUT1 are significantly expressed in the stem, and are likely to be involved in sugar loading in the phloem. MDPI 2017-10-05 /pmc/articles/PMC5664107/ /pubmed/28981454 http://dx.doi.org/10.3390/genes8100257 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Liu
Fu, Shuhua
Xu, Zhichao
Yan, Jun
Xu, Jiang
Zhou, Hong
Zhou, Jianguo
Chen, Xinlian
Li, Ying
Au, Kin Fai
Yao, Hui
Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale
title Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale
title_full Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale
title_fullStr Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale
title_full_unstemmed Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale
title_short Hybrid Sequencing of Full-Length cDNA Transcripts of Stems and Leaves in Dendrobium officinale
title_sort hybrid sequencing of full-length cdna transcripts of stems and leaves in dendrobium officinale
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664107/
https://www.ncbi.nlm.nih.gov/pubmed/28981454
http://dx.doi.org/10.3390/genes8100257
work_keys_str_mv AT heliu hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale
AT fushuhua hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale
AT xuzhichao hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale
AT yanjun hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale
AT xujiang hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale
AT zhouhong hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale
AT zhoujianguo hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale
AT chenxinlian hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale
AT liying hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale
AT aukinfai hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale
AT yaohui hybridsequencingoffulllengthcdnatranscriptsofstemsandleavesindendrobiumofficinale