Cargando…
Genome-Wide Identification and Analysis of MAPK and MAPKK Gene Families in Bread Wheat (Triticum aestivum L.)
The mitogen-activated protein kinase (MAPK) cascade is a universal signal transduction module that plays a vital role in regulating growth and development, as well as environmental stress responses in plants. Wheat is one of the most important crops worldwide. Although the MAPK kinase kinase (MAP3K)...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664134/ https://www.ncbi.nlm.nih.gov/pubmed/29053643 http://dx.doi.org/10.3390/genes8100284 |
_version_ | 1783274946586214400 |
---|---|
author | Zhan, Haoshuang Yue, Hong Zhao, Xian Wang, Meng Song, Weining Nie, Xiaojun |
author_facet | Zhan, Haoshuang Yue, Hong Zhao, Xian Wang, Meng Song, Weining Nie, Xiaojun |
author_sort | Zhan, Haoshuang |
collection | PubMed |
description | The mitogen-activated protein kinase (MAPK) cascade is a universal signal transduction module that plays a vital role in regulating growth and development, as well as environmental stress responses in plants. Wheat is one of the most important crops worldwide. Although the MAPK kinase kinase (MAP3K) family in wheat has been investigated, the MAPK and MAPK kinase (MAP2K) gene families remain unknown at present. Here, 54 MAPK and 18 MAPKK genes were identified in wheat using recent genomic information. Phylogenetic analysis of Triticum aestivum L. MAPKs and MAPKKs (TaMAPKs and TaMAPKKs) together with homologous genes from other species classified them into four groups, and the clustering was consistent with the genomic exon/intron structures. Conserved motif analysis found that MAPK proteins contained a typical TXY phosphorylation site and MAPKK proteins contained an S/T-X5-S/T motif. RNA-seq data mapping analysis showed that MAPK and MAPKK genes in group IV had tissue-specific expression profiles, whereas each group I member showed relatively high expression in all organs. Expression patterns of TaMAPK and TaMAPKK genes under stress conditions were also investigated and stress-responsive candidates were identified. Co-expression network analysis identified 11 TaMAPK genes and 6 TaMAPKK genes involved in the interaction network pathway. Overall, this study provided useful information for evolutionary and functional surveys of MAPK and MAPKK gene families in wheat and beyond. |
format | Online Article Text |
id | pubmed-5664134 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56641342017-11-06 Genome-Wide Identification and Analysis of MAPK and MAPKK Gene Families in Bread Wheat (Triticum aestivum L.) Zhan, Haoshuang Yue, Hong Zhao, Xian Wang, Meng Song, Weining Nie, Xiaojun Genes (Basel) Brief Report The mitogen-activated protein kinase (MAPK) cascade is a universal signal transduction module that plays a vital role in regulating growth and development, as well as environmental stress responses in plants. Wheat is one of the most important crops worldwide. Although the MAPK kinase kinase (MAP3K) family in wheat has been investigated, the MAPK and MAPK kinase (MAP2K) gene families remain unknown at present. Here, 54 MAPK and 18 MAPKK genes were identified in wheat using recent genomic information. Phylogenetic analysis of Triticum aestivum L. MAPKs and MAPKKs (TaMAPKs and TaMAPKKs) together with homologous genes from other species classified them into four groups, and the clustering was consistent with the genomic exon/intron structures. Conserved motif analysis found that MAPK proteins contained a typical TXY phosphorylation site and MAPKK proteins contained an S/T-X5-S/T motif. RNA-seq data mapping analysis showed that MAPK and MAPKK genes in group IV had tissue-specific expression profiles, whereas each group I member showed relatively high expression in all organs. Expression patterns of TaMAPK and TaMAPKK genes under stress conditions were also investigated and stress-responsive candidates were identified. Co-expression network analysis identified 11 TaMAPK genes and 6 TaMAPKK genes involved in the interaction network pathway. Overall, this study provided useful information for evolutionary and functional surveys of MAPK and MAPKK gene families in wheat and beyond. MDPI 2017-10-20 /pmc/articles/PMC5664134/ /pubmed/29053643 http://dx.doi.org/10.3390/genes8100284 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Zhan, Haoshuang Yue, Hong Zhao, Xian Wang, Meng Song, Weining Nie, Xiaojun Genome-Wide Identification and Analysis of MAPK and MAPKK Gene Families in Bread Wheat (Triticum aestivum L.) |
title | Genome-Wide Identification and Analysis of MAPK and MAPKK Gene Families in Bread Wheat (Triticum aestivum L.) |
title_full | Genome-Wide Identification and Analysis of MAPK and MAPKK Gene Families in Bread Wheat (Triticum aestivum L.) |
title_fullStr | Genome-Wide Identification and Analysis of MAPK and MAPKK Gene Families in Bread Wheat (Triticum aestivum L.) |
title_full_unstemmed | Genome-Wide Identification and Analysis of MAPK and MAPKK Gene Families in Bread Wheat (Triticum aestivum L.) |
title_short | Genome-Wide Identification and Analysis of MAPK and MAPKK Gene Families in Bread Wheat (Triticum aestivum L.) |
title_sort | genome-wide identification and analysis of mapk and mapkk gene families in bread wheat (triticum aestivum l.) |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664134/ https://www.ncbi.nlm.nih.gov/pubmed/29053643 http://dx.doi.org/10.3390/genes8100284 |
work_keys_str_mv | AT zhanhaoshuang genomewideidentificationandanalysisofmapkandmapkkgenefamiliesinbreadwheattriticumaestivuml AT yuehong genomewideidentificationandanalysisofmapkandmapkkgenefamiliesinbreadwheattriticumaestivuml AT zhaoxian genomewideidentificationandanalysisofmapkandmapkkgenefamiliesinbreadwheattriticumaestivuml AT wangmeng genomewideidentificationandanalysisofmapkandmapkkgenefamiliesinbreadwheattriticumaestivuml AT songweining genomewideidentificationandanalysisofmapkandmapkkgenefamiliesinbreadwheattriticumaestivuml AT niexiaojun genomewideidentificationandanalysisofmapkandmapkkgenefamiliesinbreadwheattriticumaestivuml |