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Signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis
Signal transduction and signal amplification are both important mechanisms used within biological signalling pathways. Inspired by this process, we have developed a signal amplification methodology that utilises the selectivity and high activity of enzymes in combination with the robustness and gene...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664363/ https://www.ncbi.nlm.nih.gov/pubmed/29142726 http://dx.doi.org/10.1039/c5sc01588j |
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author | Goggins, Sean Marsh, Barrie J. Lubben, Anneke T. Frost, Christopher G. |
author_facet | Goggins, Sean Marsh, Barrie J. Lubben, Anneke T. Frost, Christopher G. |
author_sort | Goggins, Sean |
collection | PubMed |
description | Signal transduction and signal amplification are both important mechanisms used within biological signalling pathways. Inspired by this process, we have developed a signal amplification methodology that utilises the selectivity and high activity of enzymes in combination with the robustness and generality of an organometallic catalyst, achieving a hybrid biological and synthetic catalyst cascade. A proligand enzyme substrate was designed to selectively self-immolate in the presence of the enzyme to release a ligand that can bind to a metal pre-catalyst and accelerate the rate of a transfer hydrogenation reaction. Enzyme-triggered catalytic signal amplification was then applied to a range of catalyst substrates demonstrating that signal amplification and signal transduction can both be achieved through this methodology. |
format | Online Article Text |
id | pubmed-5664363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-56643632017-11-15 Signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis Goggins, Sean Marsh, Barrie J. Lubben, Anneke T. Frost, Christopher G. Chem Sci Chemistry Signal transduction and signal amplification are both important mechanisms used within biological signalling pathways. Inspired by this process, we have developed a signal amplification methodology that utilises the selectivity and high activity of enzymes in combination with the robustness and generality of an organometallic catalyst, achieving a hybrid biological and synthetic catalyst cascade. A proligand enzyme substrate was designed to selectively self-immolate in the presence of the enzyme to release a ligand that can bind to a metal pre-catalyst and accelerate the rate of a transfer hydrogenation reaction. Enzyme-triggered catalytic signal amplification was then applied to a range of catalyst substrates demonstrating that signal amplification and signal transduction can both be achieved through this methodology. Royal Society of Chemistry 2015-08-01 2015-06-15 /pmc/articles/PMC5664363/ /pubmed/29142726 http://dx.doi.org/10.1039/c5sc01588j Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Goggins, Sean Marsh, Barrie J. Lubben, Anneke T. Frost, Christopher G. Signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis |
title | Signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis
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title_full | Signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis
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title_fullStr | Signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis
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title_full_unstemmed | Signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis
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title_short | Signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis
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title_sort | signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664363/ https://www.ncbi.nlm.nih.gov/pubmed/29142726 http://dx.doi.org/10.1039/c5sc01588j |
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