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Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers

The success of diffraction experiments from weakly scattering samples strongly depends on achieving an optimal signal-to-noise ratio. This is particularly important in single-particle imaging experiments where diffraction signals are typically very weak and the experiments are often accompanied by s...

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Autores principales: Wiedorn, Max O., Awel, Salah, Morgan, Andrew J., Barthelmess, Miriam, Bean, Richard, Beyerlein, Kenneth R., Chavas, Leonard M. G., Eckerskorn, Niko, Fleckenstein, Holger, Heymann, Michael, Horke, Daniel A., Knoška, Juraj, Mariani, Valerio, Oberthür, Dominik, Roth, Nils, Yefanov, Oleksandr, Barty, Anton, Bajt, Saša, Küpper, Jochen, Rode, Andrei V., Kirian, Richard A., Chapman, Henry N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665296/
https://www.ncbi.nlm.nih.gov/pubmed/29091073
http://dx.doi.org/10.1107/S1600577517011961
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author Wiedorn, Max O.
Awel, Salah
Morgan, Andrew J.
Barthelmess, Miriam
Bean, Richard
Beyerlein, Kenneth R.
Chavas, Leonard M. G.
Eckerskorn, Niko
Fleckenstein, Holger
Heymann, Michael
Horke, Daniel A.
Knoška, Juraj
Mariani, Valerio
Oberthür, Dominik
Roth, Nils
Yefanov, Oleksandr
Barty, Anton
Bajt, Saša
Küpper, Jochen
Rode, Andrei V.
Kirian, Richard A.
Chapman, Henry N.
author_facet Wiedorn, Max O.
Awel, Salah
Morgan, Andrew J.
Barthelmess, Miriam
Bean, Richard
Beyerlein, Kenneth R.
Chavas, Leonard M. G.
Eckerskorn, Niko
Fleckenstein, Holger
Heymann, Michael
Horke, Daniel A.
Knoška, Juraj
Mariani, Valerio
Oberthür, Dominik
Roth, Nils
Yefanov, Oleksandr
Barty, Anton
Bajt, Saša
Küpper, Jochen
Rode, Andrei V.
Kirian, Richard A.
Chapman, Henry N.
author_sort Wiedorn, Max O.
collection PubMed
description The success of diffraction experiments from weakly scattering samples strongly depends on achieving an optimal signal-to-noise ratio. This is particularly important in single-particle imaging experiments where diffraction signals are typically very weak and the experiments are often accompanied by significant background scattering. A simple way to tremendously reduce background scattering by placing an aperture downstream of the sample has been developed and its application in a single-particle X-ray imaging experiment at FLASH is demonstrated. Using the concept of a post-sample aperture it was possible to reduce the background scattering levels by two orders of magnitude.
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spelling pubmed-56652962017-11-09 Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers Wiedorn, Max O. Awel, Salah Morgan, Andrew J. Barthelmess, Miriam Bean, Richard Beyerlein, Kenneth R. Chavas, Leonard M. G. Eckerskorn, Niko Fleckenstein, Holger Heymann, Michael Horke, Daniel A. Knoška, Juraj Mariani, Valerio Oberthür, Dominik Roth, Nils Yefanov, Oleksandr Barty, Anton Bajt, Saša Küpper, Jochen Rode, Andrei V. Kirian, Richard A. Chapman, Henry N. J Synchrotron Radiat Laboratory Notes The success of diffraction experiments from weakly scattering samples strongly depends on achieving an optimal signal-to-noise ratio. This is particularly important in single-particle imaging experiments where diffraction signals are typically very weak and the experiments are often accompanied by significant background scattering. A simple way to tremendously reduce background scattering by placing an aperture downstream of the sample has been developed and its application in a single-particle X-ray imaging experiment at FLASH is demonstrated. Using the concept of a post-sample aperture it was possible to reduce the background scattering levels by two orders of magnitude. International Union of Crystallography 2017-10-16 /pmc/articles/PMC5665296/ /pubmed/29091073 http://dx.doi.org/10.1107/S1600577517011961 Text en © Max O. Wiedorn et al. 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Laboratory Notes
Wiedorn, Max O.
Awel, Salah
Morgan, Andrew J.
Barthelmess, Miriam
Bean, Richard
Beyerlein, Kenneth R.
Chavas, Leonard M. G.
Eckerskorn, Niko
Fleckenstein, Holger
Heymann, Michael
Horke, Daniel A.
Knoška, Juraj
Mariani, Valerio
Oberthür, Dominik
Roth, Nils
Yefanov, Oleksandr
Barty, Anton
Bajt, Saša
Küpper, Jochen
Rode, Andrei V.
Kirian, Richard A.
Chapman, Henry N.
Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers
title Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers
title_full Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers
title_fullStr Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers
title_full_unstemmed Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers
title_short Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers
title_sort post-sample aperture for low background diffraction experiments at x-ray free-electron lasers
topic Laboratory Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665296/
https://www.ncbi.nlm.nih.gov/pubmed/29091073
http://dx.doi.org/10.1107/S1600577517011961
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