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Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle foetuses

The Blonde d'Aquitaine (BA) is a French cattle breed with enhanced muscularity, partly attributable to a MSTN mutation. The BA m. Semitendinosus has a faster muscle fibre isoform phenotype comprising a higher proportion of fast type IIX fibres compared to age-matched Charolais (CH). To better u...

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Autores principales: Cassar-Malek, Isabelle, Boby, Céline, Picard, Brigitte, Reverter, Antonio, Hudson, Nicholas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665461/
https://www.ncbi.nlm.nih.gov/pubmed/28838967
http://dx.doi.org/10.1242/bio.024950
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author Cassar-Malek, Isabelle
Boby, Céline
Picard, Brigitte
Reverter, Antonio
Hudson, Nicholas J.
author_facet Cassar-Malek, Isabelle
Boby, Céline
Picard, Brigitte
Reverter, Antonio
Hudson, Nicholas J.
author_sort Cassar-Malek, Isabelle
collection PubMed
description The Blonde d'Aquitaine (BA) is a French cattle breed with enhanced muscularity, partly attributable to a MSTN mutation. The BA m. Semitendinosus has a faster muscle fibre isoform phenotype comprising a higher proportion of fast type IIX fibres compared to age-matched Charolais (CH). To better understand the molecular network of modifications in BA compared to CH muscle, we assayed the transcriptomes of the m. Semitendinosus at 110, 180, 210 and 260 days postconception (dpc). We used a combination of differential expression (DE) and regulatory impact factors (RIF) to compare and contrast muscle gene expression between the breeds. Prominently developmentally regulated genes in both breeds reflected the replacement of embryonic myosin isoforms (MYL4, MYH3) with adult isoforms (MYH1) and the upregulation of mitochondrial metabolism (CKMT2, AGXT2L1) in preparation for birth. However, the transition to a fast, glycolytic muscle phenotype in the MSTN mutant BA is detectable through downregulation of various slow twitch subunits (TNNC1, MYH7, TPM3, CSRP3) beyond 210 dpc, and a small but consistent genome-wide reduction in mRNA encoding the mitoproteome. Across the breeds, NRIP2 is the regulatory gene possessing a network change most similar to that of MSTN.
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spelling pubmed-56654612017-11-07 Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle foetuses Cassar-Malek, Isabelle Boby, Céline Picard, Brigitte Reverter, Antonio Hudson, Nicholas J. Biol Open Research Article The Blonde d'Aquitaine (BA) is a French cattle breed with enhanced muscularity, partly attributable to a MSTN mutation. The BA m. Semitendinosus has a faster muscle fibre isoform phenotype comprising a higher proportion of fast type IIX fibres compared to age-matched Charolais (CH). To better understand the molecular network of modifications in BA compared to CH muscle, we assayed the transcriptomes of the m. Semitendinosus at 110, 180, 210 and 260 days postconception (dpc). We used a combination of differential expression (DE) and regulatory impact factors (RIF) to compare and contrast muscle gene expression between the breeds. Prominently developmentally regulated genes in both breeds reflected the replacement of embryonic myosin isoforms (MYL4, MYH3) with adult isoforms (MYH1) and the upregulation of mitochondrial metabolism (CKMT2, AGXT2L1) in preparation for birth. However, the transition to a fast, glycolytic muscle phenotype in the MSTN mutant BA is detectable through downregulation of various slow twitch subunits (TNNC1, MYH7, TPM3, CSRP3) beyond 210 dpc, and a small but consistent genome-wide reduction in mRNA encoding the mitoproteome. Across the breeds, NRIP2 is the regulatory gene possessing a network change most similar to that of MSTN. The Company of Biologists Ltd 2017-08-24 /pmc/articles/PMC5665461/ /pubmed/28838967 http://dx.doi.org/10.1242/bio.024950 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Cassar-Malek, Isabelle
Boby, Céline
Picard, Brigitte
Reverter, Antonio
Hudson, Nicholas J.
Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle foetuses
title Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle foetuses
title_full Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle foetuses
title_fullStr Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle foetuses
title_full_unstemmed Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle foetuses
title_short Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle foetuses
title_sort molecular regulation of high muscle mass in developing blonde d'aquitaine cattle foetuses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665461/
https://www.ncbi.nlm.nih.gov/pubmed/28838967
http://dx.doi.org/10.1242/bio.024950
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