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Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry

INTRODUCTION: Live peripheral blood mononuclear cells (PBMCs) can be frozen and thawed for later analyses by adding and removing a cryoprotectant, such as dimethyl sulfoxide (DMSO). Laboratories across the world use various procedures, but published evidence of optimal thawing procedures is scarce....

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Detalles Bibliográficos
Autores principales: Hønge, Bo Langhoff, Petersen, Mikkel Steen, Olesen, Rikke, Møller, Bjarne Kuno, Erikstrup, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665600/
https://www.ncbi.nlm.nih.gov/pubmed/29091947
http://dx.doi.org/10.1371/journal.pone.0187440