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Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry
INTRODUCTION: Live peripheral blood mononuclear cells (PBMCs) can be frozen and thawed for later analyses by adding and removing a cryoprotectant, such as dimethyl sulfoxide (DMSO). Laboratories across the world use various procedures, but published evidence of optimal thawing procedures is scarce....
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665600/ https://www.ncbi.nlm.nih.gov/pubmed/29091947 http://dx.doi.org/10.1371/journal.pone.0187440 |