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Raman spectroscopy and regenerative medicine: a review
The field of regenerative medicine spans a wide area of the biomedical landscape—from single cell culture in laboratories to human whole-organ transplantation. To ensure that research is transferrable from bench to bedside, it is critical that we are able to assess regenerative processes in cells, t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665621/ https://www.ncbi.nlm.nih.gov/pubmed/29302348 http://dx.doi.org/10.1038/s41536-017-0014-3 |
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author | Ember, Katherine J. I. Hoeve, Marieke A. McAughtrie, Sarah L. Bergholt, Mads S. Dwyer, Benjamin J. Stevens, Molly M. Faulds, Karen Forbes, Stuart J. Campbell, Colin J. |
author_facet | Ember, Katherine J. I. Hoeve, Marieke A. McAughtrie, Sarah L. Bergholt, Mads S. Dwyer, Benjamin J. Stevens, Molly M. Faulds, Karen Forbes, Stuart J. Campbell, Colin J. |
author_sort | Ember, Katherine J. I. |
collection | PubMed |
description | The field of regenerative medicine spans a wide area of the biomedical landscape—from single cell culture in laboratories to human whole-organ transplantation. To ensure that research is transferrable from bench to bedside, it is critical that we are able to assess regenerative processes in cells, tissues, organs and patients at a biochemical level. Regeneration relies on a large number of biological factors, which can be perturbed using conventional bioanalytical techniques. A versatile, non-invasive, non-destructive technique for biochemical analysis would be invaluable for the study of regeneration; and Raman spectroscopy is a potential solution. Raman spectroscopy is an analytical method by which chemical data are obtained through the inelastic scattering of light. Since its discovery in the 1920s, physicists and chemists have used Raman scattering to investigate the chemical composition of a vast range of both liquid and solid materials. However, only in the last two decades has this form of spectroscopy been employed in biomedical research. Particularly relevant to regenerative medicine are recent studies illustrating its ability to characterise and discriminate between healthy and disease states in cells, tissue biopsies and in patients. This review will briefly outline the principles behind Raman spectroscopy and its variants, describe key examples of its applications to biomedicine, and consider areas of regenerative medicine that would benefit from this non-invasive bioanalytical tool. |
format | Online Article Text |
id | pubmed-5665621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56656212018-01-04 Raman spectroscopy and regenerative medicine: a review Ember, Katherine J. I. Hoeve, Marieke A. McAughtrie, Sarah L. Bergholt, Mads S. Dwyer, Benjamin J. Stevens, Molly M. Faulds, Karen Forbes, Stuart J. Campbell, Colin J. NPJ Regen Med Review Article The field of regenerative medicine spans a wide area of the biomedical landscape—from single cell culture in laboratories to human whole-organ transplantation. To ensure that research is transferrable from bench to bedside, it is critical that we are able to assess regenerative processes in cells, tissues, organs and patients at a biochemical level. Regeneration relies on a large number of biological factors, which can be perturbed using conventional bioanalytical techniques. A versatile, non-invasive, non-destructive technique for biochemical analysis would be invaluable for the study of regeneration; and Raman spectroscopy is a potential solution. Raman spectroscopy is an analytical method by which chemical data are obtained through the inelastic scattering of light. Since its discovery in the 1920s, physicists and chemists have used Raman scattering to investigate the chemical composition of a vast range of both liquid and solid materials. However, only in the last two decades has this form of spectroscopy been employed in biomedical research. Particularly relevant to regenerative medicine are recent studies illustrating its ability to characterise and discriminate between healthy and disease states in cells, tissue biopsies and in patients. This review will briefly outline the principles behind Raman spectroscopy and its variants, describe key examples of its applications to biomedicine, and consider areas of regenerative medicine that would benefit from this non-invasive bioanalytical tool. Nature Publishing Group UK 2017-05-15 /pmc/articles/PMC5665621/ /pubmed/29302348 http://dx.doi.org/10.1038/s41536-017-0014-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Review Article Ember, Katherine J. I. Hoeve, Marieke A. McAughtrie, Sarah L. Bergholt, Mads S. Dwyer, Benjamin J. Stevens, Molly M. Faulds, Karen Forbes, Stuart J. Campbell, Colin J. Raman spectroscopy and regenerative medicine: a review |
title | Raman spectroscopy and regenerative medicine: a review |
title_full | Raman spectroscopy and regenerative medicine: a review |
title_fullStr | Raman spectroscopy and regenerative medicine: a review |
title_full_unstemmed | Raman spectroscopy and regenerative medicine: a review |
title_short | Raman spectroscopy and regenerative medicine: a review |
title_sort | raman spectroscopy and regenerative medicine: a review |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665621/ https://www.ncbi.nlm.nih.gov/pubmed/29302348 http://dx.doi.org/10.1038/s41536-017-0014-3 |
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