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CRISPRi is not strand-specific at all loci and redefines the transcriptional landscape

CRISPRi, an adapted CRISPR-Cas9 system, is proposed to act as a strand-specific roadblock to repress transcription in eukaryotic cells using guide RNAs (sgRNAs) to target catalytically inactive Cas9 (dCas9) and offers an alternative to genetic interventions for studying pervasive antisense transcrip...

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Detalles Bibliográficos
Autores principales:	Howe, Françoise S, Russell, Andrew, Lamstaes, Anna R, El-Sagheer, Afaf, Nair, Anitha, Brown, Tom, Mellor, Jane
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2017
Materias:	Chromosomes and Gene Expression
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665645/ https://www.ncbi.nlm.nih.gov/pubmed/29058669 http://dx.doi.org/10.7554/eLife.29878

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