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Aerobic degradation of crude oil by microorganisms in soils from four geographic regions of China
A microcosm experiment was conducted for 112 d by spiking petroleum hydrocarbons into soils from four regions of China. Molecular analyses of soils from microcosms revealed changes in taxonomic diversity and oil catabolic genes of microbial communities. Degradation of total petroleum hydrocarbons (T...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665864/ https://www.ncbi.nlm.nih.gov/pubmed/29093536 http://dx.doi.org/10.1038/s41598-017-14032-5 |
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author | Liu, Qinglong Tang, Jingchun Gao, Kai Gurav, Ranjit Giesy, John P. |
author_facet | Liu, Qinglong Tang, Jingchun Gao, Kai Gurav, Ranjit Giesy, John P. |
author_sort | Liu, Qinglong |
collection | PubMed |
description | A microcosm experiment was conducted for 112 d by spiking petroleum hydrocarbons into soils from four regions of China. Molecular analyses of soils from microcosms revealed changes in taxonomic diversity and oil catabolic genes of microbial communities. Degradation of total petroleum hydrocarbons (TPHs) in Sand from the Bohai Sea (SS) and Northeast China (NE) exhibited greater microbial mineralization than those of the Dagang Oilfield (DG) and Xiamen (XM). High-throughput sequencing and denaturing gradient gel electrophoresis (DGGE) profiles demonstrated an obvious reconstruction of the bacterial community in all soils. The dominant phylum of the XM with clay soil texture was Firmicutes instead of Proteobacteria in others (DG, SS, and NE) with silty or sandy soil texture. Abundances of alkane monooxygenase gene AlkB increased by 10- to 1000-fold, relative to initial values, and were positively correlated with rates of degradation of TPHs and n-alkanes C13-C30. Abundances of naphthalene dioxygenase gene Nah were positively correlated with degradation of naphthalene and total tricyclic PAHs. Redundancy analysis (RDA) showed that abiotic process derived from geographical heterogeneity was the primary effect on bioremediation of soils contaminated with oil. The optimization of abiotic and biotic factors should be the focus of future bioremediation of oil contaminated soil. |
format | Online Article Text |
id | pubmed-5665864 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56658642017-11-08 Aerobic degradation of crude oil by microorganisms in soils from four geographic regions of China Liu, Qinglong Tang, Jingchun Gao, Kai Gurav, Ranjit Giesy, John P. Sci Rep Article A microcosm experiment was conducted for 112 d by spiking petroleum hydrocarbons into soils from four regions of China. Molecular analyses of soils from microcosms revealed changes in taxonomic diversity and oil catabolic genes of microbial communities. Degradation of total petroleum hydrocarbons (TPHs) in Sand from the Bohai Sea (SS) and Northeast China (NE) exhibited greater microbial mineralization than those of the Dagang Oilfield (DG) and Xiamen (XM). High-throughput sequencing and denaturing gradient gel electrophoresis (DGGE) profiles demonstrated an obvious reconstruction of the bacterial community in all soils. The dominant phylum of the XM with clay soil texture was Firmicutes instead of Proteobacteria in others (DG, SS, and NE) with silty or sandy soil texture. Abundances of alkane monooxygenase gene AlkB increased by 10- to 1000-fold, relative to initial values, and were positively correlated with rates of degradation of TPHs and n-alkanes C13-C30. Abundances of naphthalene dioxygenase gene Nah were positively correlated with degradation of naphthalene and total tricyclic PAHs. Redundancy analysis (RDA) showed that abiotic process derived from geographical heterogeneity was the primary effect on bioremediation of soils contaminated with oil. The optimization of abiotic and biotic factors should be the focus of future bioremediation of oil contaminated soil. Nature Publishing Group UK 2017-11-01 /pmc/articles/PMC5665864/ /pubmed/29093536 http://dx.doi.org/10.1038/s41598-017-14032-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Liu, Qinglong Tang, Jingchun Gao, Kai Gurav, Ranjit Giesy, John P. Aerobic degradation of crude oil by microorganisms in soils from four geographic regions of China |
title | Aerobic degradation of crude oil by microorganisms in soils from four geographic regions of China |
title_full | Aerobic degradation of crude oil by microorganisms in soils from four geographic regions of China |
title_fullStr | Aerobic degradation of crude oil by microorganisms in soils from four geographic regions of China |
title_full_unstemmed | Aerobic degradation of crude oil by microorganisms in soils from four geographic regions of China |
title_short | Aerobic degradation of crude oil by microorganisms in soils from four geographic regions of China |
title_sort | aerobic degradation of crude oil by microorganisms in soils from four geographic regions of china |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665864/ https://www.ncbi.nlm.nih.gov/pubmed/29093536 http://dx.doi.org/10.1038/s41598-017-14032-5 |
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