A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple...

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Autores principales: Lloyd, Yukie M., Ngati, Elise P., Salanti, Ali, Leke, Rose G. F., Taylor, Diane W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665980/
https://www.ncbi.nlm.nih.gov/pubmed/29089635
http://dx.doi.org/10.1038/s41598-017-13900-4
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author Lloyd, Yukie M.
Ngati, Elise P.
Salanti, Ali
Leke, Rose G. F.
Taylor, Diane W.
author_facet Lloyd, Yukie M.
Ngati, Elise P.
Salanti, Ali
Leke, Rose G. F.
Taylor, Diane W.
author_sort Lloyd, Yukie M.
collection PubMed
description Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.
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spelling pubmed-56659802017-11-08 A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum Lloyd, Yukie M. Ngati, Elise P. Salanti, Ali Leke, Rose G. F. Taylor, Diane W. Sci Rep Article Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized. Nature Publishing Group UK 2017-10-31 /pmc/articles/PMC5665980/ /pubmed/29089635 http://dx.doi.org/10.1038/s41598-017-13900-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lloyd, Yukie M.
Ngati, Elise P.
Salanti, Ali
Leke, Rose G. F.
Taylor, Diane W.
A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum
title A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum
title_full A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum
title_fullStr A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum
title_full_unstemmed A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum
title_short A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum
title_sort versatile, high through-put, bead-based phagocytosis assay for plasmodium falciparum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665980/
https://www.ncbi.nlm.nih.gov/pubmed/29089635
http://dx.doi.org/10.1038/s41598-017-13900-4
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