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The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels

OBJECTIVE: An experiment was conducted to study the association between the single nucleotide polymorphisms (SNPs) in 5′-untranslated regions (5′-UTR) of equine chorionic gonadotropin (eCG) genes and the serum eCG levels. METHODS: SNPs in 5′-UTR of eCG genes were screened across 10 horse breeds, inc...

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Autores principales: Liu, ShuQin, Lian, Song, Yang, YunZhou, Fu, ChunZheng, Ma, HongYing, Xiong, ZhiYao, Ling, Yao, Zhao, ChunJiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666169/
https://www.ncbi.nlm.nih.gov/pubmed/28423874
http://dx.doi.org/10.5713/ajas.17.0133
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author Liu, ShuQin
Lian, Song
Yang, YunZhou
Fu, ChunZheng
Ma, HongYing
Xiong, ZhiYao
Ling, Yao
Zhao, ChunJiang
author_facet Liu, ShuQin
Lian, Song
Yang, YunZhou
Fu, ChunZheng
Ma, HongYing
Xiong, ZhiYao
Ling, Yao
Zhao, ChunJiang
author_sort Liu, ShuQin
collection PubMed
description OBJECTIVE: An experiment was conducted to study the association between the single nucleotide polymorphisms (SNPs) in 5′-untranslated regions (5′-UTR) of equine chorionic gonadotropin (eCG) genes and the serum eCG levels. METHODS: SNPs in 5′-UTR of eCG genes were screened across 10 horse breeds, including 7 Chinese indigenous breeds and 3 imported breeds using iPLEX chemistry, and the association between the serum eCG levels of 174 pregnant Da’an mares and their serum eCG levels (determined with ELISA) was analyzed. RESULTS: Four SNPs were identified in the 5′-UTR of the eCGα gene, and one of them was unique in the indigenous breeds. There were 2 SNPs detected at the 5′ end of the eCGβ subunit gene, and one of them was only found in the Chinese breeds. The SNP g.39948246T>C at the 5′-UTR of eCGα was associated significantly with eCG levels of 75-day pregnant mare serum (p<0.05) in Da’an mares. Prediction analysis on binding sites of transcription factors showed that the g.39948246T>C mutation causes appearance of the specific binding site of hepatocyte nuclear factor 3 forkhead homolog 2 (HFH-2), which is a transcriptional repressor belonging to the forkhead protein family of transcription factors. CONCLUSION: The SNP g.39948246T>C at the 5′-UTR of eCGα is associated with eCG levels of 75-day pregnant mare serum (p<0.05).
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spelling pubmed-56661692017-12-01 The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels Liu, ShuQin Lian, Song Yang, YunZhou Fu, ChunZheng Ma, HongYing Xiong, ZhiYao Ling, Yao Zhao, ChunJiang Asian-Australas J Anim Sci Article OBJECTIVE: An experiment was conducted to study the association between the single nucleotide polymorphisms (SNPs) in 5′-untranslated regions (5′-UTR) of equine chorionic gonadotropin (eCG) genes and the serum eCG levels. METHODS: SNPs in 5′-UTR of eCG genes were screened across 10 horse breeds, including 7 Chinese indigenous breeds and 3 imported breeds using iPLEX chemistry, and the association between the serum eCG levels of 174 pregnant Da’an mares and their serum eCG levels (determined with ELISA) was analyzed. RESULTS: Four SNPs were identified in the 5′-UTR of the eCGα gene, and one of them was unique in the indigenous breeds. There were 2 SNPs detected at the 5′ end of the eCGβ subunit gene, and one of them was only found in the Chinese breeds. The SNP g.39948246T>C at the 5′-UTR of eCGα was associated significantly with eCG levels of 75-day pregnant mare serum (p<0.05) in Da’an mares. Prediction analysis on binding sites of transcription factors showed that the g.39948246T>C mutation causes appearance of the specific binding site of hepatocyte nuclear factor 3 forkhead homolog 2 (HFH-2), which is a transcriptional repressor belonging to the forkhead protein family of transcription factors. CONCLUSION: The SNP g.39948246T>C at the 5′-UTR of eCGα is associated with eCG levels of 75-day pregnant mare serum (p<0.05). Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2017-12 2017-04-19 /pmc/articles/PMC5666169/ /pubmed/28423874 http://dx.doi.org/10.5713/ajas.17.0133 Text en Copyright © 2017 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Liu, ShuQin
Lian, Song
Yang, YunZhou
Fu, ChunZheng
Ma, HongYing
Xiong, ZhiYao
Ling, Yao
Zhao, ChunJiang
The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels
title The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels
title_full The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels
title_fullStr The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels
title_full_unstemmed The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels
title_short The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels
title_sort relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666169/
https://www.ncbi.nlm.nih.gov/pubmed/28423874
http://dx.doi.org/10.5713/ajas.17.0133
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