Cargando…

The relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen

OBJECTIVE: This study aims to identify the relationship between odd- and branched-chain fatty acids (OBCFAs) and microbial nucleic acid bases in the rumen, and to establish a model to accurately predict microbial protein flow by using OBCFA. METHODS: To develop the regression equations, data on the...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Keyuan, Hao, Xiaoyan, Li, Yang, Luo, Guobin, Zhang, Yonggen, Xin, Hangshu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666194/
https://www.ncbi.nlm.nih.gov/pubmed/28728386
http://dx.doi.org/10.5713/ajas.16.0966
_version_ 1783275258668646400
author Liu, Keyuan
Hao, Xiaoyan
Li, Yang
Luo, Guobin
Zhang, Yonggen
Xin, Hangshu
author_facet Liu, Keyuan
Hao, Xiaoyan
Li, Yang
Luo, Guobin
Zhang, Yonggen
Xin, Hangshu
author_sort Liu, Keyuan
collection PubMed
description OBJECTIVE: This study aims to identify the relationship between odd- and branched-chain fatty acids (OBCFAs) and microbial nucleic acid bases in the rumen, and to establish a model to accurately predict microbial protein flow by using OBCFA. METHODS: To develop the regression equations, data on the rumen contents of individual cows were obtained from 2 feeding experiments. In the first experiment, 3 rumen-fistulated dry dairy cows arranged in a 3×3 Latin square were fed diets of differing forage to concentration ratios (F:C). The second experiment consisted of 9 lactating Holstein dairy cows of similar body weights at the same stage of pregnancy. For each lactation stage, 3 cows with similar milk production were selected. The rumen contents were sampled at 4 time points of every two hours after morning feeding 6 h, and then to analyse the concentrations of OBCFA and microbial nucleic acid bases in the rumen samples. RESULTS: The ruminal bacteria nucleic acid bases were significantly influenced by feeding diets of differing forge to concentration ratios and lactation stages of dairy cows (p<0.05). The concentrations of OBCFAs, especially odd-chain fatty acids and C15:0 isomers, strongly correlated with the microbial nucleic acid bases in the rumen (p<0.05). The equations of ruminal microbial nucleic acid bases established by ruminal OBCFAs contents showed a good predictive capacity, as indicated by reasonably low standard errors and high R-squared values. CONCLUSION: This finding suggests that the rumen OBCFA composition could be used as an internal marker of rumen microbial matter.
format Online
Article
Text
id pubmed-5666194
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
record_format MEDLINE/PubMed
spelling pubmed-56661942017-11-13 The relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen Liu, Keyuan Hao, Xiaoyan Li, Yang Luo, Guobin Zhang, Yonggen Xin, Hangshu Asian-Australas J Anim Sci Article OBJECTIVE: This study aims to identify the relationship between odd- and branched-chain fatty acids (OBCFAs) and microbial nucleic acid bases in the rumen, and to establish a model to accurately predict microbial protein flow by using OBCFA. METHODS: To develop the regression equations, data on the rumen contents of individual cows were obtained from 2 feeding experiments. In the first experiment, 3 rumen-fistulated dry dairy cows arranged in a 3×3 Latin square were fed diets of differing forage to concentration ratios (F:C). The second experiment consisted of 9 lactating Holstein dairy cows of similar body weights at the same stage of pregnancy. For each lactation stage, 3 cows with similar milk production were selected. The rumen contents were sampled at 4 time points of every two hours after morning feeding 6 h, and then to analyse the concentrations of OBCFA and microbial nucleic acid bases in the rumen samples. RESULTS: The ruminal bacteria nucleic acid bases were significantly influenced by feeding diets of differing forge to concentration ratios and lactation stages of dairy cows (p<0.05). The concentrations of OBCFAs, especially odd-chain fatty acids and C15:0 isomers, strongly correlated with the microbial nucleic acid bases in the rumen (p<0.05). The equations of ruminal microbial nucleic acid bases established by ruminal OBCFAs contents showed a good predictive capacity, as indicated by reasonably low standard errors and high R-squared values. CONCLUSION: This finding suggests that the rumen OBCFA composition could be used as an internal marker of rumen microbial matter. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2017-11 2017-06-26 /pmc/articles/PMC5666194/ /pubmed/28728386 http://dx.doi.org/10.5713/ajas.16.0966 Text en Copyright © 2017 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Liu, Keyuan
Hao, Xiaoyan
Li, Yang
Luo, Guobin
Zhang, Yonggen
Xin, Hangshu
The relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen
title The relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen
title_full The relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen
title_fullStr The relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen
title_full_unstemmed The relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen
title_short The relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen
title_sort relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666194/
https://www.ncbi.nlm.nih.gov/pubmed/28728386
http://dx.doi.org/10.5713/ajas.16.0966
work_keys_str_mv AT liukeyuan therelationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT haoxiaoyan therelationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT liyang therelationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT luoguobin therelationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT zhangyonggen therelationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT xinhangshu therelationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT liukeyuan relationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT haoxiaoyan relationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT liyang relationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT luoguobin relationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT zhangyonggen relationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen
AT xinhangshu relationshipbetweenoddandbranchedchainfattyacidsandmicrobialnucleicacidbasesinrumen