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Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin

The objective of this study was to evaluate the metabolic profile of cattle fed with or without zearalenone (ZEN) and sterigmatocystin (STC)-contaminated diets using a gas chromatography-mass spectrometry metabolomics approach. Urinary samples were collected from individual animals (n = 6 per herd)...

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Autores principales: Toda, Katsuki, Kokushi, Emiko, Uno, Seiichi, Shiiba, Ayaka, Hasunuma, Hiroshi, Fushimi, Yasuo, Wijayagunawardane, Missaka P. B., Zhang, Chunhua, Yamato, Osamu, Taniguchi, Masayasu, Fink-Gremmels, Johanna, Takagi, Mitsuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666341/
https://www.ncbi.nlm.nih.gov/pubmed/28934162
http://dx.doi.org/10.3390/toxins9100294
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author Toda, Katsuki
Kokushi, Emiko
Uno, Seiichi
Shiiba, Ayaka
Hasunuma, Hiroshi
Fushimi, Yasuo
Wijayagunawardane, Missaka P. B.
Zhang, Chunhua
Yamato, Osamu
Taniguchi, Masayasu
Fink-Gremmels, Johanna
Takagi, Mitsuhiro
author_facet Toda, Katsuki
Kokushi, Emiko
Uno, Seiichi
Shiiba, Ayaka
Hasunuma, Hiroshi
Fushimi, Yasuo
Wijayagunawardane, Missaka P. B.
Zhang, Chunhua
Yamato, Osamu
Taniguchi, Masayasu
Fink-Gremmels, Johanna
Takagi, Mitsuhiro
author_sort Toda, Katsuki
collection PubMed
description The objective of this study was to evaluate the metabolic profile of cattle fed with or without zearalenone (ZEN) and sterigmatocystin (STC)-contaminated diets using a gas chromatography-mass spectrometry metabolomics approach. Urinary samples were collected from individual animals (n = 6 per herd) from fattening female Japanese Black (JB) cattle herds (23 months old, 550–600 kg). Herd 1 had persistently high urinary ZEN and STC concentrations due to the presence of contaminated rice straw. Herd 2, the second female JB fattening herd (23 months old, 550–600 kg), received the same dietary feed as Herd 1, with non-contaminated rice straw. Urine samples were collected from Herd 1, two weeks after the contaminated rice straw was replaced with uncontaminated rice straw (Herd 1N). Identified metabolites were subjected to principal component analysis (PCA) and ANOVA. The PCA revealed that the effects on cattle metabolites depended on ZEN and STC concentrations. The contamination of cattle feed with multiple mycotoxins may alter systemic metabolic processes, including metabolites associated with ATP generation, amino acids, glycine-conjugates, organic acids, and purine bases. The results obtained from Herd 1N indicate that a two-week remedy period was not sufficient to improve the levels of urinary metabolites, suggesting that chronic contamination with mycotoxins may have long-term harmful effects on the systemic metabolism of cattle.
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spelling pubmed-56663412017-11-09 Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin Toda, Katsuki Kokushi, Emiko Uno, Seiichi Shiiba, Ayaka Hasunuma, Hiroshi Fushimi, Yasuo Wijayagunawardane, Missaka P. B. Zhang, Chunhua Yamato, Osamu Taniguchi, Masayasu Fink-Gremmels, Johanna Takagi, Mitsuhiro Toxins (Basel) Article The objective of this study was to evaluate the metabolic profile of cattle fed with or without zearalenone (ZEN) and sterigmatocystin (STC)-contaminated diets using a gas chromatography-mass spectrometry metabolomics approach. Urinary samples were collected from individual animals (n = 6 per herd) from fattening female Japanese Black (JB) cattle herds (23 months old, 550–600 kg). Herd 1 had persistently high urinary ZEN and STC concentrations due to the presence of contaminated rice straw. Herd 2, the second female JB fattening herd (23 months old, 550–600 kg), received the same dietary feed as Herd 1, with non-contaminated rice straw. Urine samples were collected from Herd 1, two weeks after the contaminated rice straw was replaced with uncontaminated rice straw (Herd 1N). Identified metabolites were subjected to principal component analysis (PCA) and ANOVA. The PCA revealed that the effects on cattle metabolites depended on ZEN and STC concentrations. The contamination of cattle feed with multiple mycotoxins may alter systemic metabolic processes, including metabolites associated with ATP generation, amino acids, glycine-conjugates, organic acids, and purine bases. The results obtained from Herd 1N indicate that a two-week remedy period was not sufficient to improve the levels of urinary metabolites, suggesting that chronic contamination with mycotoxins may have long-term harmful effects on the systemic metabolism of cattle. MDPI 2017-09-21 /pmc/articles/PMC5666341/ /pubmed/28934162 http://dx.doi.org/10.3390/toxins9100294 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Toda, Katsuki
Kokushi, Emiko
Uno, Seiichi
Shiiba, Ayaka
Hasunuma, Hiroshi
Fushimi, Yasuo
Wijayagunawardane, Missaka P. B.
Zhang, Chunhua
Yamato, Osamu
Taniguchi, Masayasu
Fink-Gremmels, Johanna
Takagi, Mitsuhiro
Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin
title Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin
title_full Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin
title_fullStr Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin
title_full_unstemmed Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin
title_short Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin
title_sort gas chromatography-mass spectrometry for metabolite profiling of japanese black cattle naturally contaminated with zearalenone and sterigmatocystin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666341/
https://www.ncbi.nlm.nih.gov/pubmed/28934162
http://dx.doi.org/10.3390/toxins9100294
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