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Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications
A challenge regarding the design of nanocarriers for drug delivery is to prevent their recognition by the immune system. To improve the blood residence time and prevent their capture by organs, nanoparticles can be designed with stealth properties using polymeric coating. In this study, we focused o...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666453/ https://www.ncbi.nlm.nih.gov/pubmed/28946628 http://dx.doi.org/10.3390/nano7100288 |
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author | Liu, Wei Chaix, Arnaud Gary-Bobo, Magali Angeletti, Bernard Masion, Armand Da Silva, Afitz Daurat, Morgane Lichon, Laure Garcia, Marcel Morère, Alain El Cheikh, Khaled Durand, Jean-Olivier Cunin, Frédérique Auffan, Mélanie |
author_facet | Liu, Wei Chaix, Arnaud Gary-Bobo, Magali Angeletti, Bernard Masion, Armand Da Silva, Afitz Daurat, Morgane Lichon, Laure Garcia, Marcel Morère, Alain El Cheikh, Khaled Durand, Jean-Olivier Cunin, Frédérique Auffan, Mélanie |
author_sort | Liu, Wei |
collection | PubMed |
description | A challenge regarding the design of nanocarriers for drug delivery is to prevent their recognition by the immune system. To improve the blood residence time and prevent their capture by organs, nanoparticles can be designed with stealth properties using polymeric coating. In this study, we focused on the influence of surface modification with polyethylene glycol and/or mannose on the stealth behavior of porous silicon nanoparticles (pSiNP, ~200 nm). In vivo biodistribution of pSiNPs formulations were evaluated in mice 5 h after intravenous injection. Results indicated that the distribution in the organs was surface functionalization-dependent. Pristine pSiNPs and PEGylated pSiNPs were distributed mainly in the liver and spleen, while mannose-functionalized pSiNPs escaped capture by the spleen, and had higher blood retention. The most efficient stealth behavior was observed with PEGylated pSiNPs anchored with mannose that were the most excreted in urine at 5 h. The biodegradation kinetics evaluated in vitro were in agreement with these in vivo observations. The biocompatibility of the pristine and functionalized pSiNPs was confirmed in vitro on human cell lines and in vivo by cytotoxic and systemic inflammation investigations, respectively. With their biocompatibility, biodegradability, and stealth properties, the pSiNPs functionalized with mannose and PEG show promising potential for biomedical applications. |
format | Online Article Text |
id | pubmed-5666453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56664532017-11-09 Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications Liu, Wei Chaix, Arnaud Gary-Bobo, Magali Angeletti, Bernard Masion, Armand Da Silva, Afitz Daurat, Morgane Lichon, Laure Garcia, Marcel Morère, Alain El Cheikh, Khaled Durand, Jean-Olivier Cunin, Frédérique Auffan, Mélanie Nanomaterials (Basel) Article A challenge regarding the design of nanocarriers for drug delivery is to prevent their recognition by the immune system. To improve the blood residence time and prevent their capture by organs, nanoparticles can be designed with stealth properties using polymeric coating. In this study, we focused on the influence of surface modification with polyethylene glycol and/or mannose on the stealth behavior of porous silicon nanoparticles (pSiNP, ~200 nm). In vivo biodistribution of pSiNPs formulations were evaluated in mice 5 h after intravenous injection. Results indicated that the distribution in the organs was surface functionalization-dependent. Pristine pSiNPs and PEGylated pSiNPs were distributed mainly in the liver and spleen, while mannose-functionalized pSiNPs escaped capture by the spleen, and had higher blood retention. The most efficient stealth behavior was observed with PEGylated pSiNPs anchored with mannose that were the most excreted in urine at 5 h. The biodegradation kinetics evaluated in vitro were in agreement with these in vivo observations. The biocompatibility of the pristine and functionalized pSiNPs was confirmed in vitro on human cell lines and in vivo by cytotoxic and systemic inflammation investigations, respectively. With their biocompatibility, biodegradability, and stealth properties, the pSiNPs functionalized with mannose and PEG show promising potential for biomedical applications. MDPI 2017-09-23 /pmc/articles/PMC5666453/ /pubmed/28946628 http://dx.doi.org/10.3390/nano7100288 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Wei Chaix, Arnaud Gary-Bobo, Magali Angeletti, Bernard Masion, Armand Da Silva, Afitz Daurat, Morgane Lichon, Laure Garcia, Marcel Morère, Alain El Cheikh, Khaled Durand, Jean-Olivier Cunin, Frédérique Auffan, Mélanie Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications |
title | Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications |
title_full | Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications |
title_fullStr | Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications |
title_full_unstemmed | Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications |
title_short | Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications |
title_sort | stealth biocompatible si-based nanoparticles for biomedical applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666453/ https://www.ncbi.nlm.nih.gov/pubmed/28946628 http://dx.doi.org/10.3390/nano7100288 |
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