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Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure

In this work, we analyze the efficiency of the modification of the implant surface. This modification was reached by the formation of a two-level relief hierarchy by means of a sol-gel approach that included dip coating with subsequent shock drying. Using this method, we fabricated a nanoporous laye...

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Autores principales: Zemtsova, Elena G., Arbenin, Andrei Y., Yudintceva, Natalia M., Valiev, Ruslan Z., Orekhov, Evgeniy V., Smirnov, Vladimir M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666488/
https://www.ncbi.nlm.nih.gov/pubmed/29027930
http://dx.doi.org/10.3390/nano7100323
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author Zemtsova, Elena G.
Arbenin, Andrei Y.
Yudintceva, Natalia M.
Valiev, Ruslan Z.
Orekhov, Evgeniy V.
Smirnov, Vladimir M.
author_facet Zemtsova, Elena G.
Arbenin, Andrei Y.
Yudintceva, Natalia M.
Valiev, Ruslan Z.
Orekhov, Evgeniy V.
Smirnov, Vladimir M.
author_sort Zemtsova, Elena G.
collection PubMed
description In this work, we analyze the efficiency of the modification of the implant surface. This modification was reached by the formation of a two-level relief hierarchy by means of a sol-gel approach that included dip coating with subsequent shock drying. Using this method, we fabricated a nanoporous layer with micron-sized defects on the nanotitanium surface. The present work continues an earlier study by our group, wherein the effect of osteoblast-like cell adhesion acceleration was found. In the present paper, we give the results of more detailed evaluation of coating efficiency. Specifically, cytological analysis was performed that included the study of the marker levels of osteoblast-like cell differentiation. We found a significant increase in the activity of alkaline phosphatase at the initial incubation stage. This is very important for implantation, since such an effect assists the decrease in the induction time of implant engraftment. Moreover, osteopontin expression remains high for long expositions. This indicates a prolonged osteogenic effect in the coating. The results suggest the acceleration of the pre-implant area mineralization and, correspondingly, the potential use of the developed coatings for bone implantation.
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spelling pubmed-56664882017-11-09 Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure Zemtsova, Elena G. Arbenin, Andrei Y. Yudintceva, Natalia M. Valiev, Ruslan Z. Orekhov, Evgeniy V. Smirnov, Vladimir M. Nanomaterials (Basel) Article In this work, we analyze the efficiency of the modification of the implant surface. This modification was reached by the formation of a two-level relief hierarchy by means of a sol-gel approach that included dip coating with subsequent shock drying. Using this method, we fabricated a nanoporous layer with micron-sized defects on the nanotitanium surface. The present work continues an earlier study by our group, wherein the effect of osteoblast-like cell adhesion acceleration was found. In the present paper, we give the results of more detailed evaluation of coating efficiency. Specifically, cytological analysis was performed that included the study of the marker levels of osteoblast-like cell differentiation. We found a significant increase in the activity of alkaline phosphatase at the initial incubation stage. This is very important for implantation, since such an effect assists the decrease in the induction time of implant engraftment. Moreover, osteopontin expression remains high for long expositions. This indicates a prolonged osteogenic effect in the coating. The results suggest the acceleration of the pre-implant area mineralization and, correspondingly, the potential use of the developed coatings for bone implantation. MDPI 2017-10-13 /pmc/articles/PMC5666488/ /pubmed/29027930 http://dx.doi.org/10.3390/nano7100323 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zemtsova, Elena G.
Arbenin, Andrei Y.
Yudintceva, Natalia M.
Valiev, Ruslan Z.
Orekhov, Evgeniy V.
Smirnov, Vladimir M.
Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure
title Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure
title_full Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure
title_fullStr Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure
title_full_unstemmed Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure
title_short Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure
title_sort bioactive coating with two-layer hierarchy of relief obtained by sol-gel method with shock drying and osteoblast response of its structure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666488/
https://www.ncbi.nlm.nih.gov/pubmed/29027930
http://dx.doi.org/10.3390/nano7100323
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