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PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles

The innate immune system consists of several complex cellular and molecular mechanisms. During inflammatory responses, blood-circulating monocytes are driven to the sites of inflammation, where they differentiate into tissue macrophages. The research of novel nanomaterials applied to biomedical scie...

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Autores principales: Gatto, Francesca, Cagliani, Roberta, Catelani, Tiziano, Guarnieri, Daniela, Moglianetti, Mauro, Pompa, Pier Paolo, Bardi, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666497/
https://www.ncbi.nlm.nih.gov/pubmed/29039753
http://dx.doi.org/10.3390/nano7100332
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author Gatto, Francesca
Cagliani, Roberta
Catelani, Tiziano
Guarnieri, Daniela
Moglianetti, Mauro
Pompa, Pier Paolo
Bardi, Giuseppe
author_facet Gatto, Francesca
Cagliani, Roberta
Catelani, Tiziano
Guarnieri, Daniela
Moglianetti, Mauro
Pompa, Pier Paolo
Bardi, Giuseppe
author_sort Gatto, Francesca
collection PubMed
description The innate immune system consists of several complex cellular and molecular mechanisms. During inflammatory responses, blood-circulating monocytes are driven to the sites of inflammation, where they differentiate into tissue macrophages. The research of novel nanomaterials applied to biomedical sciences is often limited by their toxicity or dangerous interactions with the immune cell functions. Platinum nanoparticles (PtNPs) have shown efficient antioxidant properties within several cells, but information on their potential harmful role in the monocyte-to-macrophage differentiation process is still unknown. Here, we studied the morphology and the release of cytokines in PMA-differentiated THP-1 pre-treated with 5 nm PtNPs. Although NP endocytosis was evident, we did not find differences in the cellular structure or in the release of inflammatory cytokines and chemokines compared to cells differentiated in PtNP-free medium. However, the administration of PtNPs to previously differentiated THP-1 induced massive phagocytosis of the PtNPs and a slight metabolism decrease at higher doses. Further investigation using undifferentiated and differentiated neutrophil-like HL60 confirmed the harmlessness of PtNPs with non-adherent innate immune cells. Our results demonstrate that citrate-coated PtNPs are not toxic with these immune cell lines, and do not affect the PMA-stimulated THP-1 macrophage differentiation process in vitro.
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spelling pubmed-56664972017-11-09 PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles Gatto, Francesca Cagliani, Roberta Catelani, Tiziano Guarnieri, Daniela Moglianetti, Mauro Pompa, Pier Paolo Bardi, Giuseppe Nanomaterials (Basel) Article The innate immune system consists of several complex cellular and molecular mechanisms. During inflammatory responses, blood-circulating monocytes are driven to the sites of inflammation, where they differentiate into tissue macrophages. The research of novel nanomaterials applied to biomedical sciences is often limited by their toxicity or dangerous interactions with the immune cell functions. Platinum nanoparticles (PtNPs) have shown efficient antioxidant properties within several cells, but information on their potential harmful role in the monocyte-to-macrophage differentiation process is still unknown. Here, we studied the morphology and the release of cytokines in PMA-differentiated THP-1 pre-treated with 5 nm PtNPs. Although NP endocytosis was evident, we did not find differences in the cellular structure or in the release of inflammatory cytokines and chemokines compared to cells differentiated in PtNP-free medium. However, the administration of PtNPs to previously differentiated THP-1 induced massive phagocytosis of the PtNPs and a slight metabolism decrease at higher doses. Further investigation using undifferentiated and differentiated neutrophil-like HL60 confirmed the harmlessness of PtNPs with non-adherent innate immune cells. Our results demonstrate that citrate-coated PtNPs are not toxic with these immune cell lines, and do not affect the PMA-stimulated THP-1 macrophage differentiation process in vitro. MDPI 2017-10-17 /pmc/articles/PMC5666497/ /pubmed/29039753 http://dx.doi.org/10.3390/nano7100332 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gatto, Francesca
Cagliani, Roberta
Catelani, Tiziano
Guarnieri, Daniela
Moglianetti, Mauro
Pompa, Pier Paolo
Bardi, Giuseppe
PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles
title PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles
title_full PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles
title_fullStr PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles
title_full_unstemmed PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles
title_short PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles
title_sort pma-induced thp-1 macrophage differentiation is not impaired by citrate-coated platinum nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666497/
https://www.ncbi.nlm.nih.gov/pubmed/29039753
http://dx.doi.org/10.3390/nano7100332
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