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Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells
The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. Lesions were made in human cartilage biopsies. Injured samples were pre-treated with interleukin 1β (IL1β) for 2...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666559/ https://www.ncbi.nlm.nih.gov/pubmed/29104482 http://dx.doi.org/10.7150/ijms.19835 |
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author | Sanjurjo-Rodríguez, Clara Castro-Viñuelas, Rocío Hermida-Gómez, Tamara Fuentes-Boquete, Isaac Manuel de Toro, Francisco Javier Blanco, Francisco Javier Díaz-Prado, Silvia María |
author_facet | Sanjurjo-Rodríguez, Clara Castro-Viñuelas, Rocío Hermida-Gómez, Tamara Fuentes-Boquete, Isaac Manuel de Toro, Francisco Javier Blanco, Francisco Javier Díaz-Prado, Silvia María |
author_sort | Sanjurjo-Rodríguez, Clara |
collection | PubMed |
description | The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. Lesions were made in human cartilage biopsies. Injured samples were pre-treated with interleukin 1β (IL1β) for 24 h; also, samples were not pre-treated. hBMSCs were seeded on different types of collagen scaffolds. The resulting constructs were placed into the lesions, and the biopsies were cultured for 2 months in chondrogenic medium. Using the modified ICRSII scale, neotissues from the different scaffolds showed ICRS II overall assessment scores ranging from 50% (fibrocartilage) to 100% (hyaline cartilage), except for the Col I +Col II +HS constructs (fibrocartilage/hyaline cartilage, 73%). Data showed that hBMSCs cultured only on Col I +Col II +HS scaffolds displayed a chondrocyte-like morphology and cartilage-like matrix close to native cartilage. Furthermore, IL1β pre-treated biopsies decreased capacity for repair by hBMSCs and decreased levels of chondrogenic phenotype of human cartilage lesions. |
format | Online Article Text |
id | pubmed-5666559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-56665592017-11-04 Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells Sanjurjo-Rodríguez, Clara Castro-Viñuelas, Rocío Hermida-Gómez, Tamara Fuentes-Boquete, Isaac Manuel de Toro, Francisco Javier Blanco, Francisco Javier Díaz-Prado, Silvia María Int J Med Sci Short Research Communication The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. Lesions were made in human cartilage biopsies. Injured samples were pre-treated with interleukin 1β (IL1β) for 24 h; also, samples were not pre-treated. hBMSCs were seeded on different types of collagen scaffolds. The resulting constructs were placed into the lesions, and the biopsies were cultured for 2 months in chondrogenic medium. Using the modified ICRSII scale, neotissues from the different scaffolds showed ICRS II overall assessment scores ranging from 50% (fibrocartilage) to 100% (hyaline cartilage), except for the Col I +Col II +HS constructs (fibrocartilage/hyaline cartilage, 73%). Data showed that hBMSCs cultured only on Col I +Col II +HS scaffolds displayed a chondrocyte-like morphology and cartilage-like matrix close to native cartilage. Furthermore, IL1β pre-treated biopsies decreased capacity for repair by hBMSCs and decreased levels of chondrogenic phenotype of human cartilage lesions. Ivyspring International Publisher 2017-09-28 /pmc/articles/PMC5666559/ /pubmed/29104482 http://dx.doi.org/10.7150/ijms.19835 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Short Research Communication Sanjurjo-Rodríguez, Clara Castro-Viñuelas, Rocío Hermida-Gómez, Tamara Fuentes-Boquete, Isaac Manuel de Toro, Francisco Javier Blanco, Francisco Javier Díaz-Prado, Silvia María Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells |
title | Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells |
title_full | Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells |
title_fullStr | Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells |
title_full_unstemmed | Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells |
title_short | Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells |
title_sort | human cartilage engineering in an in vitro repair model using collagen scaffolds and mesenchymal stromal cells |
topic | Short Research Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666559/ https://www.ncbi.nlm.nih.gov/pubmed/29104482 http://dx.doi.org/10.7150/ijms.19835 |
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