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MicroRNA-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin D1

MicroRNAs (miRNAs) are small non-coding RNAs that affect various biological processes by altering the expression of a target gene. An miRNA microarray analysis has previously revealed a significant decrease in miR-193a-3p levels in prostate cancer tissues compared with that in their benign prostate...

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Detalles Bibliográficos
Autores principales: Liu, Yunfu, Xu, Xin, Xu, Xianglai, Li, Shiqi, Liang, Zhen, Hu, Zhenghui, Wu, Jian, Zhu, Yi, Jin, Xiaodong, Wang, Xiao, Lin, Yiwei, Chen, Hong, Mao, Yeqing, Luo, Jindan, Zheng, Xiangyi, Xie, Liping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666665/
https://www.ncbi.nlm.nih.gov/pubmed/29142597
http://dx.doi.org/10.3892/ol.2017.6865
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author Liu, Yunfu
Xu, Xin
Xu, Xianglai
Li, Shiqi
Liang, Zhen
Hu, Zhenghui
Wu, Jian
Zhu, Yi
Jin, Xiaodong
Wang, Xiao
Lin, Yiwei
Chen, Hong
Mao, Yeqing
Luo, Jindan
Zheng, Xiangyi
Xie, Liping
author_facet Liu, Yunfu
Xu, Xin
Xu, Xianglai
Li, Shiqi
Liang, Zhen
Hu, Zhenghui
Wu, Jian
Zhu, Yi
Jin, Xiaodong
Wang, Xiao
Lin, Yiwei
Chen, Hong
Mao, Yeqing
Luo, Jindan
Zheng, Xiangyi
Xie, Liping
author_sort Liu, Yunfu
collection PubMed
description MicroRNAs (miRNAs) are small non-coding RNAs that affect various biological processes by altering the expression of a target gene. An miRNA microarray analysis has previously revealed a significant decrease in miR-193a-3p levels in prostate cancer tissues compared with that in their benign prostate hyperplasia counterparts. However, the role of miR-193a-3p has yet to be elucidated. In the present study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to evaluate the expression levels of miR-193a-3p in two human prostate cancer cell lines. Forced overexpression of miR-193a-3p was established by transfecting mimics into DU-145 and PC3 cell lines. Cell proliferation and the cell cycle were assessed using a cell viability assay, flow cytometry and a colony formation assay. In addition, the target gene of miR-193a-3p was determined by a luciferase assay, RT-qPCR and western blot analysis. The regulation of the cell cycle by miR-193a-3p was also evaluated by western blotting. The results demonstrated that miR-193a-3p expression levels were lower in prostate cancer cell lines as compared with the RWPE normal prostate epithelium cell line. Subsequent gain-of-function studies revealed that stable miR-193a-3p transfection inhibited cell viability, proliferation and colony formation, and induced G(1) phase arrest in prostate cancer cells. A luciferase assay and western blot analysis identified cyclin D1 (CCND1) as a direct target gene of miR-193a-3p. In addition, the forced expression of CCND1 was able to counter the inhibitory effects of miR-193a-3p transfection in the prostate cancer cells. In summary, the results suggest that miR-193a-3p may inhibit the viability, proliferation and survival of prostate cancer cells by regulating the expression profile of CCND1, and that miR-193a-3p may be a novel therapeutic biomarker for prostate cancer.
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spelling pubmed-56666652017-11-15 MicroRNA-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin D1 Liu, Yunfu Xu, Xin Xu, Xianglai Li, Shiqi Liang, Zhen Hu, Zhenghui Wu, Jian Zhu, Yi Jin, Xiaodong Wang, Xiao Lin, Yiwei Chen, Hong Mao, Yeqing Luo, Jindan Zheng, Xiangyi Xie, Liping Oncol Lett Articles MicroRNAs (miRNAs) are small non-coding RNAs that affect various biological processes by altering the expression of a target gene. An miRNA microarray analysis has previously revealed a significant decrease in miR-193a-3p levels in prostate cancer tissues compared with that in their benign prostate hyperplasia counterparts. However, the role of miR-193a-3p has yet to be elucidated. In the present study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to evaluate the expression levels of miR-193a-3p in two human prostate cancer cell lines. Forced overexpression of miR-193a-3p was established by transfecting mimics into DU-145 and PC3 cell lines. Cell proliferation and the cell cycle were assessed using a cell viability assay, flow cytometry and a colony formation assay. In addition, the target gene of miR-193a-3p was determined by a luciferase assay, RT-qPCR and western blot analysis. The regulation of the cell cycle by miR-193a-3p was also evaluated by western blotting. The results demonstrated that miR-193a-3p expression levels were lower in prostate cancer cell lines as compared with the RWPE normal prostate epithelium cell line. Subsequent gain-of-function studies revealed that stable miR-193a-3p transfection inhibited cell viability, proliferation and colony formation, and induced G(1) phase arrest in prostate cancer cells. A luciferase assay and western blot analysis identified cyclin D1 (CCND1) as a direct target gene of miR-193a-3p. In addition, the forced expression of CCND1 was able to counter the inhibitory effects of miR-193a-3p transfection in the prostate cancer cells. In summary, the results suggest that miR-193a-3p may inhibit the viability, proliferation and survival of prostate cancer cells by regulating the expression profile of CCND1, and that miR-193a-3p may be a novel therapeutic biomarker for prostate cancer. D.A. Spandidos 2017-11 2017-09-01 /pmc/articles/PMC5666665/ /pubmed/29142597 http://dx.doi.org/10.3892/ol.2017.6865 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Yunfu
Xu, Xin
Xu, Xianglai
Li, Shiqi
Liang, Zhen
Hu, Zhenghui
Wu, Jian
Zhu, Yi
Jin, Xiaodong
Wang, Xiao
Lin, Yiwei
Chen, Hong
Mao, Yeqing
Luo, Jindan
Zheng, Xiangyi
Xie, Liping
MicroRNA-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin D1
title MicroRNA-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin D1
title_full MicroRNA-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin D1
title_fullStr MicroRNA-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin D1
title_full_unstemmed MicroRNA-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin D1
title_short MicroRNA-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin D1
title_sort microrna-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin d1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666665/
https://www.ncbi.nlm.nih.gov/pubmed/29142597
http://dx.doi.org/10.3892/ol.2017.6865
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