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Lipidomics Unravels the Role of Leaf Lipids in Thyme Plant Response to Drought Stress

Thymus is one of the best known genera within the Labiatae (Lamiaceae) family, with more than 200 species and many medicinal and culinary uses. The effects of prolonged drought on lipid profile were investigated in tolerant and sensitive thyme plants (Thymus serpyllum L. and Thymus vulgaris L., resp...

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Autores principales: Moradi, Parviz, Mahdavi, Atiyeh, Khoshkam, Maryam, Iriti, Marcello
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666749/
https://www.ncbi.nlm.nih.gov/pubmed/28956853
http://dx.doi.org/10.3390/ijms18102067
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author Moradi, Parviz
Mahdavi, Atiyeh
Khoshkam, Maryam
Iriti, Marcello
author_facet Moradi, Parviz
Mahdavi, Atiyeh
Khoshkam, Maryam
Iriti, Marcello
author_sort Moradi, Parviz
collection PubMed
description Thymus is one of the best known genera within the Labiatae (Lamiaceae) family, with more than 200 species and many medicinal and culinary uses. The effects of prolonged drought on lipid profile were investigated in tolerant and sensitive thyme plants (Thymus serpyllum L. and Thymus vulgaris L., respectively). Non-targeted non-polar metabolite profiling was carried out using Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry with one-month-old plants exposed to drought stress, and their morpho-physiological parameters were also evaluated. Tolerant and sensitive plants exhibited clearly different responses at a physiological level. In addition, different trends for a number of non-polar metabolites were observed when comparing stressed and control samples, for both sensitive and tolerant plants. Sensitive plants showed the highest decrease (55%) in main lipid components such as galactolipids and phospholipids. In tolerant plants, the level of lipids involved in signaling increased, while intensities of those induced by stress (e.g., oxylipins) dramatically decreased (50–60%), in particular with respect to metabolites with m/z values of 519.3331, 521.3488, and 581.3709. Partial least square discriminant analysis separated all the samples into four groups: tolerant watered, tolerant stressed, sensitive watered and sensitive stressed. The combination of lipid profiling and physiological parameters represented a promising tool for investigating the mechanisms of plant response to drought stress at non-polar metabolome level.
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spelling pubmed-56667492017-11-09 Lipidomics Unravels the Role of Leaf Lipids in Thyme Plant Response to Drought Stress Moradi, Parviz Mahdavi, Atiyeh Khoshkam, Maryam Iriti, Marcello Int J Mol Sci Article Thymus is one of the best known genera within the Labiatae (Lamiaceae) family, with more than 200 species and many medicinal and culinary uses. The effects of prolonged drought on lipid profile were investigated in tolerant and sensitive thyme plants (Thymus serpyllum L. and Thymus vulgaris L., respectively). Non-targeted non-polar metabolite profiling was carried out using Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry with one-month-old plants exposed to drought stress, and their morpho-physiological parameters were also evaluated. Tolerant and sensitive plants exhibited clearly different responses at a physiological level. In addition, different trends for a number of non-polar metabolites were observed when comparing stressed and control samples, for both sensitive and tolerant plants. Sensitive plants showed the highest decrease (55%) in main lipid components such as galactolipids and phospholipids. In tolerant plants, the level of lipids involved in signaling increased, while intensities of those induced by stress (e.g., oxylipins) dramatically decreased (50–60%), in particular with respect to metabolites with m/z values of 519.3331, 521.3488, and 581.3709. Partial least square discriminant analysis separated all the samples into four groups: tolerant watered, tolerant stressed, sensitive watered and sensitive stressed. The combination of lipid profiling and physiological parameters represented a promising tool for investigating the mechanisms of plant response to drought stress at non-polar metabolome level. MDPI 2017-09-28 /pmc/articles/PMC5666749/ /pubmed/28956853 http://dx.doi.org/10.3390/ijms18102067 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moradi, Parviz
Mahdavi, Atiyeh
Khoshkam, Maryam
Iriti, Marcello
Lipidomics Unravels the Role of Leaf Lipids in Thyme Plant Response to Drought Stress
title Lipidomics Unravels the Role of Leaf Lipids in Thyme Plant Response to Drought Stress
title_full Lipidomics Unravels the Role of Leaf Lipids in Thyme Plant Response to Drought Stress
title_fullStr Lipidomics Unravels the Role of Leaf Lipids in Thyme Plant Response to Drought Stress
title_full_unstemmed Lipidomics Unravels the Role of Leaf Lipids in Thyme Plant Response to Drought Stress
title_short Lipidomics Unravels the Role of Leaf Lipids in Thyme Plant Response to Drought Stress
title_sort lipidomics unravels the role of leaf lipids in thyme plant response to drought stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666749/
https://www.ncbi.nlm.nih.gov/pubmed/28956853
http://dx.doi.org/10.3390/ijms18102067
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