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Peptides from Colochirus robustus Enhance Immune Function via Activating CD3ζ- and ZAP-70-Mediated Signaling in C57BL/6 Mice

Colochirus robustus, a species of sea cucumber, has long been used in East and Southeast Asia as nutritious food as well as for certain medicinal purpose. Studies have shown a number of biological functions associated with consumption of sea cucumber, many of which are attributed to its major compon...

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Autores principales: Du, Xiaogang, Lian, Fangliang, Li, Yunkun, Li, Dong, Wu, Dayong, Feng, Qunli, Feng, Zhijiang, Li, Yun, Bu, Guixian, Meng, Fengyan, Cao, Xiaohan, Chen, Zhiyu, Zeng, Xianyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666792/
https://www.ncbi.nlm.nih.gov/pubmed/28991187
http://dx.doi.org/10.3390/ijms18102110
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author Du, Xiaogang
Lian, Fangliang
Li, Yunkun
Li, Dong
Wu, Dayong
Feng, Qunli
Feng, Zhijiang
Li, Yun
Bu, Guixian
Meng, Fengyan
Cao, Xiaohan
Chen, Zhiyu
Zeng, Xianyin
author_facet Du, Xiaogang
Lian, Fangliang
Li, Yunkun
Li, Dong
Wu, Dayong
Feng, Qunli
Feng, Zhijiang
Li, Yun
Bu, Guixian
Meng, Fengyan
Cao, Xiaohan
Chen, Zhiyu
Zeng, Xianyin
author_sort Du, Xiaogang
collection PubMed
description Colochirus robustus, a species of sea cucumber, has long been used in East and Southeast Asia as nutritious food as well as for certain medicinal purpose. Studies have shown a number of biological functions associated with consumption of sea cucumber, many of which are attributed to its major component, sea cucumber peptides (SCP). However, how SCP impacts immune system, which is critical for host defense, has not been defined. To address this issue, in the present study, we conducted comprehensive analysis of immune function after oral administration of SCP (0, 25, 50 and 75 mg/kg body weigh) for eight weeks in C57BL/6 mice. We found that SCP treatment significantly enhanced lymphocyte proliferation, serum albumin (ALB) levels, and the natural killer (NK) cell activity. Moreover, SCP promoted functions of helper T cells (Th) as indicated by increased production of Th1 type cytokines of Interleukin (IL)-1β, IL-2, Interferon (IFN)-γ and TNF-α and Th2 type cytokines (IL-4, IL-6 and IL-10). To determine the effective components, SCP was hydrolyzed into 16 types of constituent amino acids in simulated gastrointestinal digestion and these hydrolytic amino acids (HAA) were used for the mechanistic studies in the in vitro models. Results showed that HAA enhanced lymphocyte proliferation and production of IL-2, IL-10 and IFN-γ. Furthermore, CD3ζ (CD3ζ) and ζ-chain-associated protein kinase 70 (ZAP-70), the signaling molecules essential for activating T lymphocytes, were significantly up-regulated after HAA treatment. In summary, our results suggest that SCP is effective in enhancing immune function by activating T cells via impacting CD3ζ- and ZAP-70-mediated signaling pathway.
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spelling pubmed-56667922017-11-09 Peptides from Colochirus robustus Enhance Immune Function via Activating CD3ζ- and ZAP-70-Mediated Signaling in C57BL/6 Mice Du, Xiaogang Lian, Fangliang Li, Yunkun Li, Dong Wu, Dayong Feng, Qunli Feng, Zhijiang Li, Yun Bu, Guixian Meng, Fengyan Cao, Xiaohan Chen, Zhiyu Zeng, Xianyin Int J Mol Sci Article Colochirus robustus, a species of sea cucumber, has long been used in East and Southeast Asia as nutritious food as well as for certain medicinal purpose. Studies have shown a number of biological functions associated with consumption of sea cucumber, many of which are attributed to its major component, sea cucumber peptides (SCP). However, how SCP impacts immune system, which is critical for host defense, has not been defined. To address this issue, in the present study, we conducted comprehensive analysis of immune function after oral administration of SCP (0, 25, 50 and 75 mg/kg body weigh) for eight weeks in C57BL/6 mice. We found that SCP treatment significantly enhanced lymphocyte proliferation, serum albumin (ALB) levels, and the natural killer (NK) cell activity. Moreover, SCP promoted functions of helper T cells (Th) as indicated by increased production of Th1 type cytokines of Interleukin (IL)-1β, IL-2, Interferon (IFN)-γ and TNF-α and Th2 type cytokines (IL-4, IL-6 and IL-10). To determine the effective components, SCP was hydrolyzed into 16 types of constituent amino acids in simulated gastrointestinal digestion and these hydrolytic amino acids (HAA) were used for the mechanistic studies in the in vitro models. Results showed that HAA enhanced lymphocyte proliferation and production of IL-2, IL-10 and IFN-γ. Furthermore, CD3ζ (CD3ζ) and ζ-chain-associated protein kinase 70 (ZAP-70), the signaling molecules essential for activating T lymphocytes, were significantly up-regulated after HAA treatment. In summary, our results suggest that SCP is effective in enhancing immune function by activating T cells via impacting CD3ζ- and ZAP-70-mediated signaling pathway. MDPI 2017-10-08 /pmc/articles/PMC5666792/ /pubmed/28991187 http://dx.doi.org/10.3390/ijms18102110 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Du, Xiaogang
Lian, Fangliang
Li, Yunkun
Li, Dong
Wu, Dayong
Feng, Qunli
Feng, Zhijiang
Li, Yun
Bu, Guixian
Meng, Fengyan
Cao, Xiaohan
Chen, Zhiyu
Zeng, Xianyin
Peptides from Colochirus robustus Enhance Immune Function via Activating CD3ζ- and ZAP-70-Mediated Signaling in C57BL/6 Mice
title Peptides from Colochirus robustus Enhance Immune Function via Activating CD3ζ- and ZAP-70-Mediated Signaling in C57BL/6 Mice
title_full Peptides from Colochirus robustus Enhance Immune Function via Activating CD3ζ- and ZAP-70-Mediated Signaling in C57BL/6 Mice
title_fullStr Peptides from Colochirus robustus Enhance Immune Function via Activating CD3ζ- and ZAP-70-Mediated Signaling in C57BL/6 Mice
title_full_unstemmed Peptides from Colochirus robustus Enhance Immune Function via Activating CD3ζ- and ZAP-70-Mediated Signaling in C57BL/6 Mice
title_short Peptides from Colochirus robustus Enhance Immune Function via Activating CD3ζ- and ZAP-70-Mediated Signaling in C57BL/6 Mice
title_sort peptides from colochirus robustus enhance immune function via activating cd3ζ- and zap-70-mediated signaling in c57bl/6 mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666792/
https://www.ncbi.nlm.nih.gov/pubmed/28991187
http://dx.doi.org/10.3390/ijms18102110
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