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A Temporal White Noise Analysis for Extracting the Impulse Response Function of the Human Electroretinogram
PURPOSE: We introduce a method for determining the impulse response function (IRF) of the ERG derived from responses to temporal white noise (TWN) stimuli. METHODS: This white noise ERG (wnERG) was recorded in participants with normal trichromatic vision to full-field (Ganzfeld) and 39.3° diameter f...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666911/ https://www.ncbi.nlm.nih.gov/pubmed/29109907 http://dx.doi.org/10.1167/tvst.6.6.1 |
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author | Zele, Andrew J. Feigl, Beatrix Kambhampati, Pradeep K. Aher, Avinash McKeefry, Declan Parry, Neil Maguire, John Murray, Ian Kremers, Jan |
author_facet | Zele, Andrew J. Feigl, Beatrix Kambhampati, Pradeep K. Aher, Avinash McKeefry, Declan Parry, Neil Maguire, John Murray, Ian Kremers, Jan |
author_sort | Zele, Andrew J. |
collection | PubMed |
description | PURPOSE: We introduce a method for determining the impulse response function (IRF) of the ERG derived from responses to temporal white noise (TWN) stimuli. METHODS: This white noise ERG (wnERG) was recorded in participants with normal trichromatic vision to full-field (Ganzfeld) and 39.3° diameter focal stimuli at mesopic and photopic mean luminances and at different TWN contrasts. The IRF was obtained by cross-correlating the TWN stimulus with the wnERG. RESULTS: We show that wnERG recordings are highly repeatable, with good signal-to-noise ratio, and do not lead to blink artifacts. The wnERG resembles a flash ERG waveform with an initial negativity (N1) followed by a positivity (P1), with amplitudes that are linearly related to stimulus contrast. These N1 and N1-P1 components showed commonalties in implicit times with the a- and b-waves of flash ERGs. There was a clear transition from rod- to cone-driven wnERGs at ∼1 photopic cd.m(−2). We infer that oscillatory potentials found with the flash ERG, but not the wnERG, may reflect retinal nonlinearities due to the compression of energy into a short time period during a stimulus flash. CONCLUSION: The wnERG provides a new approach to study the physiology of the retina using a stimulation method with adaptation and contrast conditions similar to natural scenes to allow for independent variation of stimulus strength and mean luminance, which is not possible with the conventional flash ERG. TRANSLATIONAL RELEVANCE: The white noise ERG methodology will be of benefit for clinical studies and animal models in the evaluation of hypotheses related to cellular redundancy to understand the effects of disease on specific visual pathways. |
format | Online Article Text |
id | pubmed-5666911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-56669112017-11-06 A Temporal White Noise Analysis for Extracting the Impulse Response Function of the Human Electroretinogram Zele, Andrew J. Feigl, Beatrix Kambhampati, Pradeep K. Aher, Avinash McKeefry, Declan Parry, Neil Maguire, John Murray, Ian Kremers, Jan Transl Vis Sci Technol Articles PURPOSE: We introduce a method for determining the impulse response function (IRF) of the ERG derived from responses to temporal white noise (TWN) stimuli. METHODS: This white noise ERG (wnERG) was recorded in participants with normal trichromatic vision to full-field (Ganzfeld) and 39.3° diameter focal stimuli at mesopic and photopic mean luminances and at different TWN contrasts. The IRF was obtained by cross-correlating the TWN stimulus with the wnERG. RESULTS: We show that wnERG recordings are highly repeatable, with good signal-to-noise ratio, and do not lead to blink artifacts. The wnERG resembles a flash ERG waveform with an initial negativity (N1) followed by a positivity (P1), with amplitudes that are linearly related to stimulus contrast. These N1 and N1-P1 components showed commonalties in implicit times with the a- and b-waves of flash ERGs. There was a clear transition from rod- to cone-driven wnERGs at ∼1 photopic cd.m(−2). We infer that oscillatory potentials found with the flash ERG, but not the wnERG, may reflect retinal nonlinearities due to the compression of energy into a short time period during a stimulus flash. CONCLUSION: The wnERG provides a new approach to study the physiology of the retina using a stimulation method with adaptation and contrast conditions similar to natural scenes to allow for independent variation of stimulus strength and mean luminance, which is not possible with the conventional flash ERG. TRANSLATIONAL RELEVANCE: The white noise ERG methodology will be of benefit for clinical studies and animal models in the evaluation of hypotheses related to cellular redundancy to understand the effects of disease on specific visual pathways. The Association for Research in Vision and Ophthalmology 2017-11-01 /pmc/articles/PMC5666911/ /pubmed/29109907 http://dx.doi.org/10.1167/tvst.6.6.1 Text en Copyright 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Articles Zele, Andrew J. Feigl, Beatrix Kambhampati, Pradeep K. Aher, Avinash McKeefry, Declan Parry, Neil Maguire, John Murray, Ian Kremers, Jan A Temporal White Noise Analysis for Extracting the Impulse Response Function of the Human Electroretinogram |
title | A Temporal White Noise Analysis for Extracting the Impulse Response Function of the Human Electroretinogram |
title_full | A Temporal White Noise Analysis for Extracting the Impulse Response Function of the Human Electroretinogram |
title_fullStr | A Temporal White Noise Analysis for Extracting the Impulse Response Function of the Human Electroretinogram |
title_full_unstemmed | A Temporal White Noise Analysis for Extracting the Impulse Response Function of the Human Electroretinogram |
title_short | A Temporal White Noise Analysis for Extracting the Impulse Response Function of the Human Electroretinogram |
title_sort | temporal white noise analysis for extracting the impulse response function of the human electroretinogram |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666911/ https://www.ncbi.nlm.nih.gov/pubmed/29109907 http://dx.doi.org/10.1167/tvst.6.6.1 |
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